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04106nam a22003375i 4500 |
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20211028161207.0 |
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cr nn 008mamaa |
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100301s2009 xxu| s |||| 0|eng d |
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|a 9781603270151
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| 024 |
7 |
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|a 10.1007/978-1-60327-015-1
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a DNA-Protein Interactions :
|b Principles and Protocols, Third Edition /
|c edited by Tom Moss, Benoît Leblanc.
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| 250 |
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|a 3rd ed. 2009.
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| 260 |
# |
# |
|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2009.
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| 300 |
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|a XVIII, 680 p. 131 illus. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 543
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| 505 |
0 |
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|a Filter-Binding Assays -- Electrophoretic Mobility Shift Assays for the Analysis of DNA-Protein Interactions -- DNase I Footprinting -- Exonuclease III Footprinting on Immobilized DNA Templates -- Hydroxyl Radical Footprinting of Protein-DNA Complexes -- The Use of Diethyl Pyrocarbonate and Potassium Permanganate as Probes for Strand Separation and Structural Distortions in DNA -- Uranyl Photofootprinting -- Identification of Protein/DNA Contacts with Dimethyl Sulfate: Methylation Protection and Methylation Interference -- Ethylation Interference Footprinting of DNA-Protein Complexes -- Site-Directed Cleavage of DNA by Protein-Fe(II) EDTA Conjugates Within Model Chromatin Complexes -- Identification of Nucleic Acid High-Affinity Binding Sequences of Proteins by SELEX -- Identification of Sequence-Specific DNA-Binding Proteins by Southwestern Blotting -- Footprinting DNA-Protein Interactions in Native Polyacrylamide Gels by Chemical Nucleolytic Activity of 1,10-Phenanthroline-Copper -- Determination of a Transcription Factor-Binding Site by Nuclease Protection Footprinting onto Southwestern Blots -- Use of a Reporter Gene Assay in Yeast for Genetic Analysis of DNA-Protein Interactions -- Chromatin Immunoprecipitation in Mammalian Cells -- Sequential Chromatin Immunoprecipitation Protocol: ChIP-reChIP -- Profiling Genome-Wide Histone Modifications and Variants by ChIP-chip on Tiling Microarrays in S. cerevisiae -- Nucleosome Mapping -- In Cellulo DNA Analysis (LMPCR Footprinting) -- Atomic Force Microscopy Imaging and Probing of DNA, Proteins, and Protein-DNA Complexes: Silatrane Surface Chemistry -- Two-Dimensional Crystallisation of Soluble Protein Complexes -- Assays for Transcription Factor Activity -- Ultraviolet Crosslinking of DNA-Protein Complexes via 8-Azidoadenine -- Static and Kinetic Site-Specific Protein-DNA Photocrosslinking: Analysis of Bacterial Transcription Initiation Complexes -- Use of Site-Specific Protein-DNA Photocrosslinking of Purified Complexes to Analyze the Topology of the RNA Polymerase II Transcription Initiation Complex -- Site-Directed DNA Crosslinking of Large Multisubunit Protein-DNA Complexes -- Functional Studies of DNA-Protein Interactions Using FRET Techniques -- Single-Molecule FRET Analysis of Protein-DNA Complexes -- Analysis of DNA Supercoiling Induced by DNA-Protein Interactions -- The Cruciform DNA Mobility Shift Assay: A Tool to Study Proteins that Recognize Bent DNA -- Plasmid Vectors for the Analysis of Protein-Induced DNA Bending -- Analysis of Distant Communication on Defined Chromatin Templates In Vitro -- A Competition Assay for DNA Binding Using the Fluorescent Probe ANS -- Fluorescence Spectroscopy and Anisotropy in the Analysis of DNA-Protein Interactions -- Circular Dichroism for the Analysis of Protein-DNA Interactions -- Defining the Thermodynamics of Protein/DNA Complexes and Their Components Using Micro-calorimetry -- Surface Plasmon Resonance Assays of DNA-Protein Interactions.
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| 650 |
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0 |
|a Proteins .
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| 650 |
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0 |
|a Human genetics.
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| 650 |
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0 |
|a Proteomics.
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| 650 |
1 |
4 |
|a Protein Science.
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| 650 |
2 |
4 |
|a Human Genetics.
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| 650 |
2 |
4 |
|a Proteomics.
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| 700 |
1 |
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|a Moss, Tom.
|e editor.
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| 700 |
1 |
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|a Leblanc, Benoît.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
|