DNA-Protein Interactions : Principles and Protocols, Third Edition /
| Autor Corporativo: | |
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| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2009.
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| Edición: | 3rd ed. 2009. |
| Colección: | Methods in Molecular Biology,
543 |
| Materias: |
Tabla de Contenidos:
- Filter-Binding Assays
- Electrophoretic Mobility Shift Assays for the Analysis of DNA-Protein Interactions
- DNase I Footprinting
- Exonuclease III Footprinting on Immobilized DNA Templates
- Hydroxyl Radical Footprinting of Protein-DNA Complexes
- The Use of Diethyl Pyrocarbonate and Potassium Permanganate as Probes for Strand Separation and Structural Distortions in DNA
- Uranyl Photofootprinting
- Identification of Protein/DNA Contacts with Dimethyl Sulfate: Methylation Protection and Methylation Interference
- Ethylation Interference Footprinting of DNA-Protein Complexes
- Site-Directed Cleavage of DNA by Protein-Fe(II) EDTA Conjugates Within Model Chromatin Complexes
- Identification of Nucleic Acid High-Affinity Binding Sequences of Proteins by SELEX
- Identification of Sequence-Specific DNA-Binding Proteins by Southwestern Blotting
- Footprinting DNA-Protein Interactions in Native Polyacrylamide Gels by Chemical Nucleolytic Activity of 1,10-Phenanthroline-Copper
- Determination of a Transcription Factor-Binding Site by Nuclease Protection Footprinting onto Southwestern Blots
- Use of a Reporter Gene Assay in Yeast for Genetic Analysis of DNA-Protein Interactions
- Chromatin Immunoprecipitation in Mammalian Cells
- Sequential Chromatin Immunoprecipitation Protocol: ChIP-reChIP
- Profiling Genome-Wide Histone Modifications and Variants by ChIP-chip on Tiling Microarrays in S. cerevisiae
- Nucleosome Mapping
- In Cellulo DNA Analysis (LMPCR Footprinting)
- Atomic Force Microscopy Imaging and Probing of DNA, Proteins, and Protein-DNA Complexes: Silatrane Surface Chemistry
- Two-Dimensional Crystallisation of Soluble Protein Complexes
- Assays for Transcription Factor Activity
- Ultraviolet Crosslinking of DNA-Protein Complexes via 8-Azidoadenine
- Static and Kinetic Site-Specific Protein-DNA Photocrosslinking: Analysis of Bacterial Transcription Initiation Complexes
- Use of Site-Specific Protein-DNA Photocrosslinking of Purified Complexes to Analyze the Topology of the RNA Polymerase II Transcription Initiation Complex
- Site-Directed DNA Crosslinking of Large Multisubunit Protein-DNA Complexes
- Functional Studies of DNA-Protein Interactions Using FRET Techniques
- Single-Molecule FRET Analysis of Protein-DNA Complexes
- Analysis of DNA Supercoiling Induced by DNA-Protein Interactions
- The Cruciform DNA Mobility Shift Assay: A Tool to Study Proteins that Recognize Bent DNA
- Plasmid Vectors for the Analysis of Protein-Induced DNA Bending
- Analysis of Distant Communication on Defined Chromatin Templates In Vitro
- A Competition Assay for DNA Binding Using the Fluorescent Probe ANS
- Fluorescence Spectroscopy and Anisotropy in the Analysis of DNA-Protein Interactions
- Circular Dichroism for the Analysis of Protein-DNA Interactions
- Defining the Thermodynamics of Protein/DNA Complexes and Their Components Using Micro-calorimetry
- Surface Plasmon Resonance Assays of DNA-Protein Interactions.