Genetic Modification of Hematopoietic Stem Cells : Methods and Protocols /
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| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2009.
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| Edition: | 1st ed. 2009. |
| Series: | Methods in Molecular Biology,
506 |
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Table of Contents:
- Immunomagnetic Enrichment of Human and Mouse Hematopoietic Stem Cells for Gene Therapy Applications
- Isolation of Human and Mouse Hematopoietic Stem Cells
- Murine Hematopoietic Stem Cell Transduction Using Retroviral Vectors
- Genetic Modification of Human Hematopoietic Cells: Preclinical Optimization of Oncoretroviral-mediated Gene Transfer for Clinical Trials
- Short-Term Culture of Human CD34+ Cells for Lentiviral Gene Transfer
- T Cell Culture for Gammaretroviral Transfer
- Retroviral Transduction of Murine Primary T Lymphocytes
- Lentiviral Vector Gene Transfer into Human T Cells
- DNA Transposons for Modification of Human Primary T Lymphocytes
- Retroviral Gene Transfer into Primary Human Natural Killer Cells
- Lentiviral Vector-Mediated Genetic Programming of Mouse and Human Dendritic Cells
- In Situ (In Vivo) Gene Transfer into Murine Bone Marrow Stem Cells
- In Vivo and Ex Vivo Gene Transfer in Thymocytes and Thymocyte Precursors
- Design and Production of Retro- and Lentiviral Vectors for Gene Expression in Hematopoietic Cells
- Knock-Down of Gene Expression in Hematopoietic Cells
- The Use of Retroviral Vectors for tet-Regulated Gene Expression in Cell Populations
- Detection of Replication Competent Retrovirus and Lentivirus
- Release Testing of Retroviral Vectors and Gene-Modified Cells
- Copy Number Determination of Genetically-Modified Hematopoietic Stem Cells
- Tissue Procurement for Molecular Studies Using Laser-Assisted Microdissection
- Leukemia Diagnosis in Murine Bone Marrow Transplantation Models
- Humanized Mouse Models to Study the Human Haematopoietic Stem Cell Compartment
- Canine Models of Gene-Modified Hematopoiesis
- Detection of Retroviral Integration Sites by Linear Amplification-Mediated PCR and Tracking of Individual Integration Clones in Different Samples
- Retroviral Insertion Site Analysis in Dominant Haematopoietic Clones
- Tracking Gene-Modified T Cells In Vivo
- DNA Microarray Studies of Hematopoietic Subpopulations
- Quantification of Genomic Mutations in Murine Hematopoietic Cells
- Proteomics Studies After Hematopoietic Stem Cell Transplantation
- Spectral Karyotyping and Fluorescence In Situ Hybridization of Murine Cells
- Database Setup for Preclinical Studies of Gene-Modified Hematopoiesis
- The US and EU Regulatory Perspectives on the Clinical Use of Hematopoietic Stem/Progenitor Cells Genetically Modified Ex Vivo by Retroviral Vectors.