Enzyme Engineering : Methods and Protocols /
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| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2013.
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| Edition: | 1st ed. 2013. |
| Series: | Methods in Molecular Biology,
978 |
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Table of Contents:
- A Tripartite Fusion System for the Selection of Protein Variants with Increased Stability in vivo
- Determining Enzyme Kinetics via Isothermal Titration Calorimetry
- GFP Reporter Screens for the Engineering of Amino Acid Degrading Enzymes from Libraries Expressed in Bacteria
- Flow Cytometric Assays for Interrogating LAGLIDADG Homing Endonuclease DNA Binding and Cleavage Properties
- TAL Effector Nuclease (TALEN) Engineering
- In vitro Evolution of Enzymes
- Residue-Specific Incorporation of Unnatural Amino Acids into Proteins In vitro and In vivo
- Reconstructing Evolutionary Adaptive Paths for Protein Engineering
- Oligonucleotide Recombination Enabled Site-Specific Mutagenesis in Bacteria
- FX Cloning: A Versatile High-Throughput Cloning System for Characterization of Enzyme Variants
- Use of Sulfolobus solfataricus PCNA Subunit Proteins to Direct the Assembly of Multimeric Enzyme Complexes
- Gene Synthesis by Assembly of Deoxyuridine Containing Oligonucleotides
- Protein Engineering: Single or Multiple Site-Directed Mutagenesis
- Gene Assembly and Combinatorial Libraries in S. cerevisiae via Reiterative Recombination
- Promiscuity-Based Enzyme Selection for Rational Directed Evolution Experiments
- Rational Protein Sequence Diversification by Multi-Codon Scanning Mutagenesis
- Screening Libraries for Improved Solubility: Using E. coli Dihydrofolate Reductase as a Reporter
- In Vitro Directed Evolution of Enzymes Expressed by E. coli in Micro-Titre Plates.