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100301s2006 xxu| s |||| 0|eng d |
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|a 9781597451444
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| 024 |
7 |
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|a 10.1385/1597451444
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
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|a Dictyostelium discoideum Protocols /
|c edited by Ludwig Eichinger.
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| 250 |
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|a 1st ed. 2006.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2006.
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| 300 |
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|a XVI, 564 p. 186 illus. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 346
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| 505 |
0 |
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|a The Organism, Community Resources, and Genome-wide Studies -- The Secret Lives of Dictyostelium -- The Genome of Dictyostelium discoideum -- The cDNA Sequencing Project -- dictyBase and the Dicty Stock Center -- Analysis of Gene Expression Using cDNA Microarrays -- Proteomic Analysis of Dictyostelium discoideum -- Basic Methods and Molecular Genetic Techniques -- Cultivation, Spore Production, and Mating -- Parasexual Genetics Using Axenic Cells -- Slug Phototaxis, Thermotaxis, and Spontaneous Turning Behavior -- Purification Techniques of Subcellular Compartments for Analytical and Preparative Purposes -- Generation of Multiple Knockout Mutants Using the Cre-loxP System -- Restriction Enzyme-Mediated Integration (REMI) Mutagenesis -- RNA Interference and Antisense-Mediated Gene Silencing in Dictyostelium -- Imaging and Localization Methods -- Application of Fluorescent Protein Tags as Reporters in Live-Cell Imaging Studies -- Investigating Gene Expression -- Application of 2D and 3D DIAS to Motion Analysis of Live Cells in Transmission and Confocal Microscopy Imaging -- Using Quantitative Fluorescence Microscopy and FRET Imaging to Measure Spatiotemporal Signaling Events in Single Living Cells -- Visualizing Signaling and Cell Movement During the Multicellular Stages of Dictyostelium Development -- Under-Agarose Chemotaxis of Dictyostelium discoideum -- Optimized Fixation and Immunofluorescence Staining Methods for Dictyostelium Cells -- Cryofixation Methods for Ultrastructural Studies of Dictyostelium discoideum -- Dictyostelium as Model Organism -- Analysis of Signal Transduction -- Assaying Chemotaxis of Dictyostelium Cells -- Characterization of Cross-Linked Actin Filament Gels and Bundles Using Birefringence and Polarized Light Scattering -- Quantitative and Microscopic Methods for Studying the Endocytic Pathway -- Preparation of Intact, Highly Purified Phagosomes from Dictyostelium -- Assaying Cell-Cell Adhesion -- Periodic Activation of ERK2 and Partial Involvement of G Protein in ERK2 Activation by cAMP in Dictyostelium Cells -- An Improved Method for Dictyostelium Centrosome Isolation -- Epiggenetics in Dictyostelium -- Dictyostelium discoideum as a Model to Study Host-Pathogen Interactions -- Pharmacogenetics -- How to Assess and Study Cell Death in Dictyostelium discoideum.
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| 650 |
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|a Biochemistry.
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| 650 |
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|a Cell biology.
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| 650 |
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4 |
|a Biochemistry, general.
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| 650 |
2 |
4 |
|a Cell Biology.
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| 700 |
1 |
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|a Eichinger, Ludwig.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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