Dictyostelium discoideum Protocols /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2006.
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| Edición: | 1st ed. 2006. |
| Colección: | Methods in Molecular Biology,
346 |
| Materias: |
Tabla de Contenidos:
- The Organism, Community Resources, and Genome-wide Studies
- The Secret Lives of Dictyostelium
- The Genome of Dictyostelium discoideum
- The cDNA Sequencing Project
- dictyBase and the Dicty Stock Center
- Analysis of Gene Expression Using cDNA Microarrays
- Proteomic Analysis of Dictyostelium discoideum
- Basic Methods and Molecular Genetic Techniques
- Cultivation, Spore Production, and Mating
- Parasexual Genetics Using Axenic Cells
- Slug Phototaxis, Thermotaxis, and Spontaneous Turning Behavior
- Purification Techniques of Subcellular Compartments for Analytical and Preparative Purposes
- Generation of Multiple Knockout Mutants Using the Cre-loxP System
- Restriction Enzyme-Mediated Integration (REMI) Mutagenesis
- RNA Interference and Antisense-Mediated Gene Silencing in Dictyostelium
- Imaging and Localization Methods
- Application of Fluorescent Protein Tags as Reporters in Live-Cell Imaging Studies
- Investigating Gene Expression
- Application of 2D and 3D DIAS to Motion Analysis of Live Cells in Transmission and Confocal Microscopy Imaging
- Using Quantitative Fluorescence Microscopy and FRET Imaging to Measure Spatiotemporal Signaling Events in Single Living Cells
- Visualizing Signaling and Cell Movement During the Multicellular Stages of Dictyostelium Development
- Under-Agarose Chemotaxis of Dictyostelium discoideum
- Optimized Fixation and Immunofluorescence Staining Methods for Dictyostelium Cells
- Cryofixation Methods for Ultrastructural Studies of Dictyostelium discoideum
- Dictyostelium as Model Organism
- Analysis of Signal Transduction
- Assaying Chemotaxis of Dictyostelium Cells
- Characterization of Cross-Linked Actin Filament Gels and Bundles Using Birefringence and Polarized Light Scattering
- Quantitative and Microscopic Methods for Studying the Endocytic Pathway
- Preparation of Intact, Highly Purified Phagosomes from Dictyostelium
- Assaying Cell-Cell Adhesion
- Periodic Activation of ERK2 and Partial Involvement of G Protein in ERK2 Activation by cAMP in Dictyostelium Cells
- An Improved Method for Dictyostelium Centrosome Isolation
- Epiggenetics in Dictyostelium
- Dictyostelium discoideum as a Model to Study Host-Pathogen Interactions
- Pharmacogenetics
- How to Assess and Study Cell Death in Dictyostelium discoideum.