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171007s2017 xxu| s |||| 0|eng d |
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|a 9781493972319
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| 024 |
7 |
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|a 10.1007/978-1-4939-7231-9
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a Functional Genomics :
|b Methods and Protocols /
|c edited by Michael Kaufmann, Claudia Klinger, Andreas Savelsbergh.
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| 250 |
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|a 3rd ed. 2017.
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| 260 |
# |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2017.
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| 300 |
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|a XV, 392 p. 74 illus., 51 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 1654
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| 505 |
0 |
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|a Predicting RNA Structure with Vfold -- RNA Function Prediction -- Computational Prediction of Novel miRNAs from Genome-Wide Data -- Protein Structure Modeling with MODELLER -- Protein Function Prediction -- Capturing Three-Dimensional Genome Organization in Individual Cells by Single-Cell Hi-C -- Genome-Wide Cell Type-Specific Mapping of In Vivo Chromatin Protein Binding Using an FLP-Inducible DamID System in Drosophila -- DNA Methylation Profiling Using Long-Read Single-Molecule Real-Time Bisulfite Sequencing (SMRT-BS) -- Copy Number Variation Analysis by Droplet Digital PCR -- MicroScale Thermophoresis: A Rapid and Precise Method to Quantify Protein-Nucleic Acid Interactions in Solution -- Establishment of the CRISPR/Cas9 System for Targeted Gene Disruption and Gene Tagging -- Holistic and Affordable Analyses of MicroRNA Expression Profiles Using Tagged cDNA Libraries and a Multiplex Sequencing Strategy -- MicroRNA Expression Analysis Using Small RNA Sequencing Discovery and RT-qPCR-Based Validation -- Using Firefly® Particle Technology for Multiplex MicroRNA Profiling Without RNA Purification -- Multiplex Real-Time PCR Using Encoded Microparticles for MicroRNA Profiling -- Optimized Whole Transcriptome Profiling of Motor Axons -- 2D-DIGE in Proteomics -- STAGE-Diging in Proteomics -- Protein Arrays I: Antibody Arrays -- Protein Arrays II: Antigen Arrays -- Protein Arrays III: Reverse-Phase Protein Arrays -- Isolation of Exosomes for the Purpose of Protein Cargo Analysis with the Use of Mass Spectrometry -- Virus-Induced Gene Silencing (VIGS) and Foreign Gene Expression in Pisum sativum L. Using the ‘One-Step’ Bean pod mottle virus (BPMV) Viral Vector -- Re-Expressing Epigenetically Silenced Genes by Inducing DNA Demethylation through Targeting of Ten-Eleven Translocation 2 to Any Given Genomic Locus -- Knockdown of Rice MicroRNA166 by Short Tandem Target Mimic (STTM) -- RNAi-Mediated Knockdown of Protein Expression -- Engineered Zinc Finger DNA-Binding Domains: Synthesis, Assessment of DNA-Binding Affinity, and Direct Protein Delivery to Mammalian Cells -- Production, Purification, and Titration of First-Generation Adenovirus Vectors.
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| 650 |
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0 |
|a Human genetics.
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| 650 |
1 |
4 |
|a Human Genetics.
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| 700 |
1 |
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|a Kaufmann, Michael.
|e editor.
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| 700 |
1 |
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|a Klinger, Claudia.
|e editor.
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| 700 |
1 |
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|a Savelsbergh, Andreas.
|e editor.
|
| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
|