Functional Genomics : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | , , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer New York : Imprint: Humana,
2017.
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| Edición: | 3rd ed. 2017. |
| Colección: | Methods in Molecular Biology,
1654 |
| Materias: |
Tabla de Contenidos:
- Predicting RNA Structure with Vfold
- RNA Function Prediction
- Computational Prediction of Novel miRNAs from Genome-Wide Data
- Protein Structure Modeling with MODELLER
- Protein Function Prediction
- Capturing Three-Dimensional Genome Organization in Individual Cells by Single-Cell Hi-C
- Genome-Wide Cell Type-Specific Mapping of In Vivo Chromatin Protein Binding Using an FLP-Inducible DamID System in Drosophila
- DNA Methylation Profiling Using Long-Read Single-Molecule Real-Time Bisulfite Sequencing (SMRT-BS)
- Copy Number Variation Analysis by Droplet Digital PCR
- MicroScale Thermophoresis: A Rapid and Precise Method to Quantify Protein-Nucleic Acid Interactions in Solution
- Establishment of the CRISPR/Cas9 System for Targeted Gene Disruption and Gene Tagging
- Holistic and Affordable Analyses of MicroRNA Expression Profiles Using Tagged cDNA Libraries and a Multiplex Sequencing Strategy
- MicroRNA Expression Analysis Using Small RNA Sequencing Discovery and RT-qPCR-Based Validation
- Using Firefly® Particle Technology for Multiplex MicroRNA Profiling Without RNA Purification
- Multiplex Real-Time PCR Using Encoded Microparticles for MicroRNA Profiling
- Optimized Whole Transcriptome Profiling of Motor Axons
- 2D-DIGE in Proteomics
- STAGE-Diging in Proteomics
- Protein Arrays I: Antibody Arrays
- Protein Arrays II: Antigen Arrays
- Protein Arrays III: Reverse-Phase Protein Arrays
- Isolation of Exosomes for the Purpose of Protein Cargo Analysis with the Use of Mass Spectrometry
- Virus-Induced Gene Silencing (VIGS) and Foreign Gene Expression in Pisum sativum L. Using the ‘One-Step’ Bean pod mottle virus (BPMV) Viral Vector
- Re-Expressing Epigenetically Silenced Genes by Inducing DNA Demethylation through Targeting of Ten-Eleven Translocation 2 to Any Given Genomic Locus
- Knockdown of Rice MicroRNA166 by Short Tandem Target Mimic (STTM)
- RNAi-Mediated Knockdown of Protein Expression
- Engineered Zinc Finger DNA-Binding Domains: Synthesis, Assessment of DNA-Binding Affinity, and Direct Protein Delivery to Mammalian Cells
- Production, Purification, and Titration of First-Generation Adenovirus Vectors.