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02482nam a22003255i 4500 |
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20211029082411.0 |
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cr nn 008mamaa |
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100301s2008 xxu| s |||| 0|eng d |
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|a 9781597455831
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| 024 |
7 |
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|a 10.1007/978-1-59745-583-1
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a Drosophila :
|b Methods and Protocols /
|c edited by Christian Dahmann.
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| 250 |
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|a 1st ed. 2008.
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| 260 |
# |
# |
|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2008.
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| 300 |
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|a XII, 438 p. 101 illus., 13 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 420
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| 505 |
0 |
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|a Drosophila melanogaster and the Development of Biology in the 20th Century -- Getting Started -- FlyBase -- The Use of P-Element Transposons to Generate Transgenic Flies -- The GAL4 System -- P-Element Mutagenesis -- EMS Screens -- RNAi Screening in Cultured Drosophila Cells -- Methods for Homologous Recombination in Drosophila -- Recombinases and Their Use in Gene Activation, Gene Inactivation, and Transgenesis -- Cuticle Preparation of Drosophila Embryos and Larvae -- Immunolabeling of Embryos -- Imaging Cellular and Molecular Dynamics in Live Embryos Using Fluorescent Proteins -- Analysis of Neuromuscular Junctions -- Immunolabeling of Imaginal Discs -- Imaging Drosophila Pupal Wing Morphogenesis -- Microscopic Analysis of the Adult Drosophila Retina Using Semithin Plastic Sections -- Fluorescent In Situ Hybridization Protocols in Drosophila Embryos and Tissues -- Linear RNA Amplification for the Production of Microarray Hybridization Probes -- An Overview of the Identification, Detection, and Functional Analysis of Drosophila MicroRNAs -- Extraction and Immunoblotting of Proteins From Embryos -- Purification of Drosophila Protein Complexes for Mass Spectrometry -- Mass Production of Drosophila Embryos and Chromatographic Purification of Native Protein Complexes -- Flow Cytometric Analysis of Drosophila Cells -- Drosophila Cell Lines as Model Systems and as an Experimental Tool.
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| 650 |
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0 |
|a Entomology.
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| 650 |
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0 |
|a Cell biology.
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| 650 |
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0 |
|a Animal genetics.
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| 650 |
1 |
4 |
|a Entomology.
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| 650 |
2 |
4 |
|a Cell Biology.
|
| 650 |
2 |
4 |
|a Animal Genetics and Genomics.
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| 700 |
1 |
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|a Dahmann, Christian.
|e editor.
|
| 710 |
2 |
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|a SpringerLink (Online service)
|
| 773 |
0 |
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|t Springer eBooks
|