Drosophila : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2008.
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| Edición: | 1st ed. 2008. |
| Colección: | Methods in Molecular Biology,
420 |
| Materias: |
Tabla de Contenidos:
- Drosophila melanogaster and the Development of Biology in the 20th Century
- Getting Started
- FlyBase
- The Use of P-Element Transposons to Generate Transgenic Flies
- The GAL4 System
- P-Element Mutagenesis
- EMS Screens
- RNAi Screening in Cultured Drosophila Cells
- Methods for Homologous Recombination in Drosophila
- Recombinases and Their Use in Gene Activation, Gene Inactivation, and Transgenesis
- Cuticle Preparation of Drosophila Embryos and Larvae
- Immunolabeling of Embryos
- Imaging Cellular and Molecular Dynamics in Live Embryos Using Fluorescent Proteins
- Analysis of Neuromuscular Junctions
- Immunolabeling of Imaginal Discs
- Imaging Drosophila Pupal Wing Morphogenesis
- Microscopic Analysis of the Adult Drosophila Retina Using Semithin Plastic Sections
- Fluorescent In Situ Hybridization Protocols in Drosophila Embryos and Tissues
- Linear RNA Amplification for the Production of Microarray Hybridization Probes
- An Overview of the Identification, Detection, and Functional Analysis of Drosophila MicroRNAs
- Extraction and Immunoblotting of Proteins From Embryos
- Purification of Drosophila Protein Complexes for Mass Spectrometry
- Mass Production of Drosophila Embryos and Chromatographic Purification of Native Protein Complexes
- Flow Cytometric Analysis of Drosophila Cells
- Drosophila Cell Lines as Model Systems and as an Experimental Tool.