DNA'Protein Interactions : Principles and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2001.
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| Edición: | 2nd ed. 2001. |
| Colección: | Methods in Molecular Biology,
148 |
| Materias: |
Tabla de Contenidos:
- Filter-Binding Assays
- Electrophoretic Mobility Shift Assays for the Analysis of DNA-Protein Interactions
- DNase I Footprinting
- Footprinting with Exonuclease III
- Hydroxyl Radical Footprinting
- The Use of Diethyl Pyrocarbonate and Potassium Permanganate as Probes for Strand Separation and Structural Distortions in DNA
- Footprinting DNA-Protein Interactions in Native Polyacrylamide Gels by Chemical Nucleolytic Activity of 1,10-Phenanthroline-Copper
- Uranyl Photofootprinting
- Osmium Tetroxide Modification and the Study of DNA-Protein Interactions
- Determination of a Transcription-Factor-Binding Site by Nuclease Protection Footprinting onto Southwestern Blots
- Diffusible Singlet Oxygen as a Probe of DNA Deformation
- Ultraviolet-Laser Footprinting
- In Vivo DNA Analysis
- Identification of Protein-DNA Contacts with Dimethyl Sulfate
- Ethylation Interference
- Hydroxyl Radical Interference
- Identification of Sequence-Specific DNA-Binding Proteins by Southwestern Blotting
- A Competition Assay for DNA Binding Using the Fluorescent Probe ANS
- Site-Directed Cleavage of DNA by Linker Histone-Fe(II) EDTA Conjugates
- Nitration of Tyrosine Residues in Protein-Nucleic Acid Complexes
- Chemical Modification of Lysine by Reductive Methylation
- Limited Proteolysis of Protein-Nucleic Acid Complexes
- Ultraviolet Crosslinking of DNA-Protein Complexes via 8-Azidoadenine
- Site-Specific Protein-DNA Photocrosslinking
- Site-Directed DNA Photoaffinity Labeling of RNA Polymerase III Transcription Complexes
- Use of Site-Specific Protein-DNA Photocrosslinking to Analyze the Molecular Organization of the RNA Polymerase II Initiation Complex
- UV Laser-Induced Protein-DNA Crosslinking
- Plasmid Vectors for the Analysis of Protein-Induced DNA Bending
- Engineering Nucleic Acid-Binding Proteins by Phage Display
- Genetic Analysis of DNA-Protein Interactions Using a Reporter Gene Assay in Yeast
- Assays for Transcription Factor Activity
- Assay of Restriction Endonucleases Using Oligonucleotides
- Analysis of DNA-Protein Interactions by Intrinsic Fluorescence
- Circular Dichroism for the Analysis of Protein-DNA Interactions
- Calorimetry of Protein-DNA Complexes and Their Components
- Surface Plasmon Resonance Applied to DNA-Protein Complexes
- Reconstitution of Protein-DNA Complexes for Crystallization
- Two-Dimensional Crystallization of Soluble Protein Complexes
- Atomic Force Microscopy of DNA and Protein-DNA Complexes Using Functionalized Mica Substrates
- Electron Microscopy of Protein-Nucleic Acid Complexes
- Scanning Transmission Electron Microscopy of DNA-Protein Complexes
- Determination of Nucleic Acid Recognition Sequences by SELEX
- High DNA-Protein Crosslinking Yield with Two-Wavelength Femtosecond Laser Irradiation.