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190412s2019 xxu| s |||| 0|eng d |
| 020 |
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|a 9781493992102
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| 024 |
7 |
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|a 10.1007/978-1-4939-9210-2
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a Leishmania :
|b Methods and Protocols /
|c edited by Joachim Clos.
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| 250 |
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|a 1st ed. 2019.
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| 260 |
# |
# |
|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2019.
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| 300 |
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|a XIII, 370 p. 77 illus., 60 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 1971
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| 505 |
0 |
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|a Host-Free Systems for Differentiation of Axenic Leishmania -- Phylogenetic Studies -- A Guide to Next Generation Sequence Analysis of Leishmania Genomes -- Quantitative RNA Analysis using RNA Seq -- Ribosome Profiling in Trypanosomatids -- Cosmid Library Construction and Functional Cloning -- Cos-Seq, A High Throughput Gain of Function Screen for Drug Resistance Studies in Leishmania -- Gene Replacement by Homologous Recombination -- LeishGEdit: A Method for Rapid Gene Knockout and Tagging using CRISPR-Cas9 -- DiCre-Based Inducible Disruption of Leishmania Genes -- DiCre-Based Inducible Gene Expression -- Generation of Bone Marrow-Derived Macrophages for In Vitro Infection Experiments -- Quantification of Intracellular Leishmania spp. Using Real-Time Quantitative PCR (qPCR) -- In Vitro Infections of Macrophage-Like Cell Lines with Leishmania infantum for Drug Screening -- Quantification of Parasite Loads by Automated Microscopic Image Analysis -- Quantification of Leishmania Parasites in Murine Models of Visceral Infection -- Syrian Hamster as an Advanced Experimental Model for Visceral Leishmaniasis -- Experimental Cutaneous Leishmaniasis: Mouse Models for Resolution of Inflammation versus Chronicity of Disease -- Establishment, Maintenance of Phlemotomus spp. In the Laboratory, and Infection with Leishmania spp.
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| 650 |
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0 |
|a Infectious diseases.
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| 650 |
1 |
4 |
|a Infectious Diseases.
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| 700 |
1 |
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|a Clos, Joachim.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 900 |
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|a Libro descargado a ALEPH en bloque (proveniente de proveedor)
|