Leishmania : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer New York : Imprint: Humana,
2019.
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| Edición: | 1st ed. 2019. |
| Colección: | Methods in Molecular Biology,
1971 |
| Materias: |
Tabla de Contenidos:
- Host-Free Systems for Differentiation of Axenic Leishmania
- Phylogenetic Studies
- A Guide to Next Generation Sequence Analysis of Leishmania Genomes
- Quantitative RNA Analysis using RNA Seq
- Ribosome Profiling in Trypanosomatids
- Cosmid Library Construction and Functional Cloning
- Cos-Seq, A High Throughput Gain of Function Screen for Drug Resistance Studies in Leishmania
- Gene Replacement by Homologous Recombination
- LeishGEdit: A Method for Rapid Gene Knockout and Tagging using CRISPR-Cas9
- DiCre-Based Inducible Disruption of Leishmania Genes
- DiCre-Based Inducible Gene Expression
- Generation of Bone Marrow-Derived Macrophages for In Vitro Infection Experiments
- Quantification of Intracellular Leishmania spp. Using Real-Time Quantitative PCR (qPCR)
- In Vitro Infections of Macrophage-Like Cell Lines with Leishmania infantum for Drug Screening
- Quantification of Parasite Loads by Automated Microscopic Image Analysis
- Quantification of Leishmania Parasites in Murine Models of Visceral Infection
- Syrian Hamster as an Advanced Experimental Model for Visceral Leishmaniasis
- Experimental Cutaneous Leishmaniasis: Mouse Models for Resolution of Inflammation versus Chronicity of Disease
- Establishment, Maintenance of Phlemotomus spp. In the Laboratory, and Infection with Leishmania spp.