Hypertension : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Touyz, Rhian M. (Editor ), Schiffrin, Ernesto L. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2017.
Edición:1st ed. 2017.
Colección:Methods in Molecular Biology, 1527
Materias:
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245 1 0 |a Hypertension :  |b Methods and Protocols /  |c edited by Rhian M. Touyz, Ernesto L. Schiffrin. 
250 |a 1st ed. 2017. 
260 # # |a New York, NY :  |b Springer New York :  |b Imprint: Humana,  |c 2017. 
300 |a XV, 437 p. 78 illus., 40 illus. in color. :  |b online resource. 
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490 1 |a Methods in Molecular Biology,  |v 1527 
505 0 |a Large Scale Transcriptome Analysis -- Methods to Assess Genetic Risk Prediction -- Microarray Analysis of Hypertension -- Tissue Proteomics in Vascular Disease -- Urine Metabolomics in Hypertension Research -- Systems Biology Approach in Hypertension Research -- Measurement of Angiotensin Peptides: HPLC, RIA -- Measurement of Angiotensin Converting Enzyme 2 Activity in Biological Fluid (ACE2) -- Determining the Enzymatic Activity of Angiotensin-Converting Enzyme 2 (ACE2) in Brain Tissue and Cerebrospinal Fluid Using a Quenched Fluorescent Substrate -- Measurement of Cardiac Angiotensin II by Immunoassays, HPLC-chip/Mass Spectrometry and Functional Assays -- Analysis of the Aldosterone Synthase (CYP11B2) and 11b-Hydroxylase (CYP11B1) Genes -- Dopaminergic Immunofluorescence Studies in Kidney Tissue -- Techniques for the Evaluation of the Genetic Expression, Intracellular Storage and Secretion of Polypeptide Hormones with Special Reference to the Natriuretic Peptides (Nps) -- Intracellular Free Calcium Measurement Using Confocal Imaging -- Measuring T-type Calcium Channel Currents in Isolated Vascular Smooth Muscle Cells -- In Vitro Analysis of Hypertensive Signal Transduction: Kinase Activation, Kinase Manipulation, and Physiologic Outputs -- In vitro and in vivo Approaches to Assess Rho-kinase Activity -- NADPH Oxidases and Measurement of Reactive Oxygen Species -- Measurement of Superoxide Production and NADPH Oxidase Activity by HPLC Analysis of Dihydroethidium Oxidation -- Assessment of Caveolae/Lipid Rafts in Isolated Cells -- Isolation and Characterization of Circulating Microparticles by Flow Cytometry -- Isolation of Mature Adipocytes from White Adipose Tissue and Gene Expression Studies by Real-Time Quantitative RT-PCR -- Isolation and Differentiation of Murine Macrophages -- Isolation and Differentiation of Human Macrophages -- Isolation of Immune Cells for Adoptive Transfer -- Isolation and Culture of Endothelial Cells from Large Vessels -- Isolation and Culture of Vascular Smooth Muscle Cells from Small and Large Vessels -- Evaluation of Endothelial Dysfunction in vivo -- Vascular Reactive of Isolated Aorta To Study the Angiotensin-(1-7) Actions -- Generation of a Mouse Model with Smooth Muscle Cell Specific Loss of the Expression of< PPARγ -- Renal Delivery of Anti-microRNA Oligonucleotides in Rats -- In Vivo Analysis of Hypertension: Induction of Hypertension, In Vivo Kinase Manipulation And Assessment Of Physiologic Outputs. . 
650 0 |a Molecular biology. 
650 1 4 |a Molecular Medicine. 
700 1 |a Touyz, Rhian M.  |e editor. 
700 1 |a Schiffrin, Ernesto L.  |e editor. 
710 2 |a SpringerLink (Online service) 
773 0 |t Springer eBooks 
900 |a Libro descargado a ALEPH en bloque (proveniente de proveedor)