Hypertension : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Touyz, Rhian M. (Editor ), Schiffrin, Ernesto L. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2017.
Edición:1st ed. 2017.
Colección:Methods in Molecular Biology, 1527
Materias:
Tabla de Contenidos:
  • Large Scale Transcriptome Analysis
  • Methods to Assess Genetic Risk Prediction
  • Microarray Analysis of Hypertension
  • Tissue Proteomics in Vascular Disease
  • Urine Metabolomics in Hypertension Research
  • Systems Biology Approach in Hypertension Research
  • Measurement of Angiotensin Peptides: HPLC, RIA
  • Measurement of Angiotensin Converting Enzyme 2 Activity in Biological Fluid (ACE2)
  • Determining the Enzymatic Activity of Angiotensin-Converting Enzyme 2 (ACE2) in Brain Tissue and Cerebrospinal Fluid Using a Quenched Fluorescent Substrate
  • Measurement of Cardiac Angiotensin II by Immunoassays, HPLC-chip/Mass Spectrometry and Functional Assays
  • Analysis of the Aldosterone Synthase (CYP11B2) and 11b-Hydroxylase (CYP11B1) Genes
  • Dopaminergic Immunofluorescence Studies in Kidney Tissue
  • Techniques for the Evaluation of the Genetic Expression, Intracellular Storage and Secretion of Polypeptide Hormones with Special Reference to the Natriuretic Peptides (Nps)
  • Intracellular Free Calcium Measurement Using Confocal Imaging
  • Measuring T-type Calcium Channel Currents in Isolated Vascular Smooth Muscle Cells
  • In Vitro Analysis of Hypertensive Signal Transduction: Kinase Activation, Kinase Manipulation, and Physiologic Outputs
  • In vitro and in vivo Approaches to Assess Rho-kinase Activity
  • NADPH Oxidases and Measurement of Reactive Oxygen Species
  • Measurement of Superoxide Production and NADPH Oxidase Activity by HPLC Analysis of Dihydroethidium Oxidation
  • Assessment of Caveolae/Lipid Rafts in Isolated Cells
  • Isolation and Characterization of Circulating Microparticles by Flow Cytometry
  • Isolation of Mature Adipocytes from White Adipose Tissue and Gene Expression Studies by Real-Time Quantitative RT-PCR
  • Isolation and Differentiation of Murine Macrophages
  • Isolation and Differentiation of Human Macrophages
  • Isolation of Immune Cells for Adoptive Transfer
  • Isolation and Culture of Endothelial Cells from Large Vessels
  • Isolation and Culture of Vascular Smooth Muscle Cells from Small and Large Vessels
  • Evaluation of Endothelial Dysfunction in vivo
  • Vascular Reactive of Isolated Aorta To Study the Angiotensin-(1-7) Actions
  • Generation of a Mouse Model with Smooth Muscle Cell Specific Loss of the Expression of< PPARγ
  • Renal Delivery of Anti-microRNA Oligonucleotides in Rats
  • In Vivo Analysis of Hypertension: Induction of Hypertension, In Vivo Kinase Manipulation And Assessment Of Physiologic Outputs. .