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100301s2009 xxu| s |||| 0|eng d |
| 020 |
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|a 9781597451703
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| 024 |
7 |
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|a 10.1007/978-1-59745-170-3
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
|
| 245 |
1 |
0 |
|a HIV Protocols :
|b Second Edition /
|c edited by Vinayaka R. Prasad, Ganjam V. Kalpana.
|
| 250 |
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|a 2nd ed. 2009.
|
| 260 |
# |
# |
|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2009.
|
| 300 |
|
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|a XIV, 457 p. :
|b online resource.
|
| 336 |
|
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 485
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| 505 |
0 |
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|a Preparation of Virus Particles and their Analysis -- Methods for Viral RNA Isolation and PCR Amplification for Sequencing of Near Full-Length HIV-1 Genomes -- Purification of HIV-1 Virions by Subtilisin Digestion or CD45 Immunoaffinity Depletion for Biochemical Studies -- Calculating HIV-1 Infectious Titre Using a Virtual TCID50 Method -- Methods To Study Hiv-1 Replication -- Cell-Free Assays for HIV-1 Uncoating -- Real-Time PCR Analysis of HIV-1 Replication Post-entry Events -- Analysis of 2-LTR Circle Junctions of Viral DNA in Infected Cells -- HIV-1 Recombination: An Experimental Assay and a Phylogenetic Approach -- Methods of Preparation and Analysis of Intracellular Reverse Transcription Complexes -- Analysis of Viral and Cellular Proteins in HIV-1 Reverse Transcription Complexes by Co-immunoprecipitation -- Isolation and Analysis of HIV-1 Preintegration Complexes -- Bisarsenical Labeling of HIV-1 for Real-Time Fluorescence Microscopy -- Methods for the Study of HIV-1 Assembly -- Assembly of Immature HIV-1 Capsids Using a Cell-Free System -- Preparation of Recombinant HIV-1 Gag Protein and Assembly of Virus-Like Particles In Vitro -- Methods for the Analysis of HIV-1 Nucleocapsid Protein Interactions with Oligonucleotides -- Methods for Analysis of Incorporation and Annealing Methods for Analysis of tRNALys in HIV-1 -- Specialized Approaches To Study Hiv-1 Biology and Pathogenesis -- Somatic Cell Genetic Analyses to Identify HIV-1 Host Restriction Factors -- Rapid, Controlled and Intensive Lentiviral Vector-Based RNAi -- Reverse Two-Hybrid Screening to Analyze Protein–Protein Interaction of HIV-1 Viral and Cellular Proteins -- Methods to Study Monocyte Migration Induced by HIV-Infected Cells -- Novel Mouse Models for Understanding HIV-1 Pathogenesis -- Immunological Studies Of Hiv -- Mucosal Antibody Responses to HIV -- Isolating Mucosal Lymphocytes from Biopsy Tissue for Cellular Immunology Assays -- Quantifying HIV-1-Specific CD8 + T-Cell Responses Using ELISPOT and Cytokine Flow Cytometry -- Multiparameter Flow Cytometry Monitoring of T Cell Responses -- Measuring HIV Neutralization in a Luciferase Reporter Gene Assay -- Assessing the Antiviral Activity of HIV-1-Specific Cytotoxic T Lymphocytes -- Methods for Quantitating Antigen-Specific T Cell Responses Using Functional Assays in Rhesus Macaques -- Drug Resistant Viruses And Viral Evolution -- Isolation of Drug-Resistant Mutant HIV Variants Using Tissue Culture Drug Selection -- Virus Evolution as a Tool to Study HIV-1 Biology.
|
| 650 |
|
0 |
|a Infectious diseases.
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| 650 |
|
0 |
|a Immunology.
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| 650 |
|
0 |
|a Virology.
|
| 650 |
1 |
4 |
|a Infectious Diseases.
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| 650 |
2 |
4 |
|a Immunology.
|
| 650 |
2 |
4 |
|a Virology.
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| 700 |
1 |
|
|a Prasad, Vinayaka R.
|e editor.
|
| 700 |
1 |
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|a Kalpana, Ganjam V.
|e editor.
|
| 710 |
2 |
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|a SpringerLink (Online service)
|
| 773 |
0 |
|
|t Springer eBooks
|