HIV Protocols : Second Edition /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Prasad, Vinayaka R. (Editor ), Kalpana, Ganjam V. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2009.
Edición:2nd ed. 2009.
Colección:Methods in Molecular Biology, 485
Materias:
Tabla de Contenidos:
  • Preparation of Virus Particles and their Analysis
  • Methods for Viral RNA Isolation and PCR Amplification for Sequencing of Near Full-Length HIV-1 Genomes
  • Purification of HIV-1 Virions by Subtilisin Digestion or CD45 Immunoaffinity Depletion for Biochemical Studies
  • Calculating HIV-1 Infectious Titre Using a Virtual TCID50 Method
  • Methods To Study Hiv-1 Replication
  • Cell-Free Assays for HIV-1 Uncoating
  • Real-Time PCR Analysis of HIV-1 Replication Post-entry Events
  • Analysis of 2-LTR Circle Junctions of Viral DNA in Infected Cells
  • HIV-1 Recombination: An Experimental Assay and a Phylogenetic Approach
  • Methods of Preparation and Analysis of Intracellular Reverse Transcription Complexes
  • Analysis of Viral and Cellular Proteins in HIV-1 Reverse Transcription Complexes by Co-immunoprecipitation
  • Isolation and Analysis of HIV-1 Preintegration Complexes
  • Bisarsenical Labeling of HIV-1 for Real-Time Fluorescence Microscopy
  • Methods for the Study of HIV-1 Assembly
  • Assembly of Immature HIV-1 Capsids Using a Cell-Free System
  • Preparation of Recombinant HIV-1 Gag Protein and Assembly of Virus-Like Particles In Vitro
  • Methods for the Analysis of HIV-1 Nucleocapsid Protein Interactions with Oligonucleotides
  • Methods for Analysis of Incorporation and Annealing Methods for Analysis of tRNALys in HIV-1
  • Specialized Approaches To Study Hiv-1 Biology and Pathogenesis
  • Somatic Cell Genetic Analyses to Identify HIV-1 Host Restriction Factors
  • Rapid, Controlled and Intensive Lentiviral Vector-Based RNAi
  • Reverse Two-Hybrid Screening to Analyze Protein–Protein Interaction of HIV-1 Viral and Cellular Proteins
  • Methods to Study Monocyte Migration Induced by HIV-Infected Cells
  • Novel Mouse Models for Understanding HIV-1 Pathogenesis
  • Immunological Studies Of Hiv
  • Mucosal Antibody Responses to HIV
  • Isolating Mucosal Lymphocytes from Biopsy Tissue for Cellular Immunology Assays
  • Quantifying HIV-1-Specific CD8 + T-Cell Responses Using ELISPOT and Cytokine Flow Cytometry
  • Multiparameter Flow Cytometry Monitoring of T Cell Responses
  • Measuring HIV Neutralization in a Luciferase Reporter Gene Assay
  • Assessing the Antiviral Activity of HIV-1-Specific Cytotoxic T Lymphocytes
  • Methods for Quantitating Antigen-Specific T Cell Responses Using Functional Assays in Rhesus Macaques
  • Drug Resistant Viruses And Viral Evolution
  • Isolation of Drug-Resistant Mutant HIV Variants Using Tissue Culture Drug Selection
  • Virus Evolution as a Tool to Study HIV-1 Biology.