HIV Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Prasad, Vinayaka R. (Editor ), Kalpana, Ganjam V. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2016.
Edición:3rd ed. 2016.
Colección:Methods in Molecular Biology, 1354
Materias:
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020 |a 9781493930463 
024 7 |a 10.1007/978-1-4939-3046-3  |2 doi 
040 |a Sistema de Bibliotecas del Tecnológico de Costa Rica 
245 1 0 |a HIV Protocols /  |c edited by Vinayaka R. Prasad, Ganjam V. Kalpana. 
250 |a 3rd ed. 2016. 
260 # # |a New York, NY :  |b Springer New York :  |b Imprint: Humana,  |c 2016. 
300 |a XIV, 383 p. 63 illus., 36 illus. in color. :  |b online resource. 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
338 |a online resource  |b cr  |2 rdacarrier 
490 1 |a Methods in Molecular Biology,  |v 1354 
505 0 |a Quantifying CD4/CCR5 Usage Efficiency of HIV-1 ENV Using the Affinofile System -- Measuring T Cell-to-T Cell HIV-1 Transfer, Viral Fusion, and Infection Using Flow Cytometry -- HIV-1 Capsid Stabilization Assay -- Detection and Tracking of Dual-Labeled HIV Particles Using Wide-Field Live Cell Imaging to Follow Viral Core Integrity -- HIV-1 Reverse Transcriptase Based Assay to Determine Cellular dNTP Concentrations -- Rapid Determination of HIV-1 Mutant Frequencies and Mutation Spectra Using an mCherry/EGFP Dual Reporter Viral Vector -- Novel Biochemical Tools for Probing HIV RNA Structure -- Analysis of HIV-1 Gag-RNA Interactions in Cells and Virions by CLIP-seq -- Isolation of Cognate Cellular and Viral Ribonucleoprotein Complexes of HIV-1 RNA Applicable to Proteomic Discovery and Molecular Investigations -- Methods for the Analyses of Inhibitor-Induced Aberrant Multimerization of HIV-1 Integrase -- Quantification of HIV-1 Gag Localization Within Virus Producer Cells -- Methods to Study Determinants for Membrane Targeting of HIV-1 Gag In Vitro -- Visualizing the Behavior of HIV-Infected T Cells In Vivo Using Multiphoton Intravital Microscopy -- Modeling HIV-1 Mucosal Transmission and Prevention in Humanized Mice -- High Throughput Humanized Mouse Models for Evaluation of HIV-1 Therapeutics and Pathogenesis -- Measuring the Frequency of Latent HIV-1 in Resting CD4+ T Cells Using a Limiting Dilution Co-Culture Assay -- LGIT In Vitro Latency Model in Primary and T Cell Lines to Test HIV-1 Reactivation Compounds -- Improved Methods to Detect Low Levels of HIV Using Antibody-Based Technologies -- Analysis of ABCA1 and Cholesterol Efflux in HIV-Infected Cells -- The Proteomic Characterization of Plasma or Serum from HIV-Infected Patients -- Proteomic Characterization of Exosomes from HIV-1-Infected Cells -- Detecting HIV-1 Tat in Cell Culture Supernatants by ELISA or Western Blot -- Protocol for Detection of HIV-Tat Protein in Cerebrospinal Fluid by a Sandwich Enzyme Linked Immunosorbent Assay -- Measuring the Uptake and Transactivation Function of HIV-1 Tat Protein in a Trans-Cellular Co-Cultivation Set Up -- Evaluating the Role of Viral Proteins in HIV-Mediated Neurotoxicity Using Primary Human Neuronal Cultures. 
650 0 |a Immunology. 
650 0 |a Virology. 
650 1 4 |a Immunology. 
650 2 4 |a Virology. 
700 1 |a Prasad, Vinayaka R.  |e editor. 
700 1 |a Kalpana, Ganjam V.  |e editor. 
710 2 |a SpringerLink (Online service) 
773 0 |t Springer eBooks