HIV Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Prasad, Vinayaka R. (Editor ), Kalpana, Ganjam V. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2016.
Edición:3rd ed. 2016.
Colección:Methods in Molecular Biology, 1354
Materias:
Tabla de Contenidos:
  • Quantifying CD4/CCR5 Usage Efficiency of HIV-1 ENV Using the Affinofile System
  • Measuring T Cell-to-T Cell HIV-1 Transfer, Viral Fusion, and Infection Using Flow Cytometry
  • HIV-1 Capsid Stabilization Assay
  • Detection and Tracking of Dual-Labeled HIV Particles Using Wide-Field Live Cell Imaging to Follow Viral Core Integrity
  • HIV-1 Reverse Transcriptase Based Assay to Determine Cellular dNTP Concentrations
  • Rapid Determination of HIV-1 Mutant Frequencies and Mutation Spectra Using an mCherry/EGFP Dual Reporter Viral Vector
  • Novel Biochemical Tools for Probing HIV RNA Structure
  • Analysis of HIV-1 Gag-RNA Interactions in Cells and Virions by CLIP-seq
  • Isolation of Cognate Cellular and Viral Ribonucleoprotein Complexes of HIV-1 RNA Applicable to Proteomic Discovery and Molecular Investigations
  • Methods for the Analyses of Inhibitor-Induced Aberrant Multimerization of HIV-1 Integrase
  • Quantification of HIV-1 Gag Localization Within Virus Producer Cells
  • Methods to Study Determinants for Membrane Targeting of HIV-1 Gag In Vitro
  • Visualizing the Behavior of HIV-Infected T Cells In Vivo Using Multiphoton Intravital Microscopy
  • Modeling HIV-1 Mucosal Transmission and Prevention in Humanized Mice
  • High Throughput Humanized Mouse Models for Evaluation of HIV-1 Therapeutics and Pathogenesis
  • Measuring the Frequency of Latent HIV-1 in Resting CD4+ T Cells Using a Limiting Dilution Co-Culture Assay
  • LGIT In Vitro Latency Model in Primary and T Cell Lines to Test HIV-1 Reactivation Compounds
  • Improved Methods to Detect Low Levels of HIV Using Antibody-Based Technologies
  • Analysis of ABCA1 and Cholesterol Efflux in HIV-Infected Cells
  • The Proteomic Characterization of Plasma or Serum from HIV-Infected Patients
  • Proteomic Characterization of Exosomes from HIV-1-Infected Cells
  • Detecting HIV-1 Tat in Cell Culture Supernatants by ELISA or Western Blot
  • Protocol for Detection of HIV-Tat Protein in Cerebrospinal Fluid by a Sandwich Enzyme Linked Immunosorbent Assay
  • Measuring the Uptake and Transactivation Function of HIV-1 Tat Protein in a Trans-Cellular Co-Cultivation Set Up
  • Evaluating the Role of Viral Proteins in HIV-Mediated Neurotoxicity Using Primary Human Neuronal Cultures.