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02297nam a22003015i 4500 |
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20201006090708.0 |
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cr nn 008mamaa |
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171124s2018 xxu| s |||| 0|eng d |
| 020 |
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|a 9781493975198
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| 024 |
7 |
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|a 10.1007/978-1-4939-7519-8
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Innate Immune Activation :
|b Methods and Protocols /
|c edited by Dominic De Nardo, Christine M De Nardo.
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| 250 |
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|a 1st ed. 2018.
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| 260 |
# |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2018.
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| 300 |
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|a XIII, 251 p. 54 illus., 40 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 1714
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| 505 |
0 |
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|a Emerging concepts in innate immunity -- Bioinformatic Assessment of macrophage activation by the innate immune system -- Generation of Genetic Knockouts in myeloid cell lines using a lentiviral CRISPR/Cas9 system -- Modeling primary human monocytes with the trans-differentiation cell line BLaER1 -- Measurement of NF-κB activation in TLR-activated macrophages -- Biochemical isolation of the myddosome from murine macrophages -- Generation of innate immune reporter cell lines using retroviral transduction -- Myddosome Formation by Luminescence-based Mammalian Interactome Mapping (LUMIER) -- Inflammatory caspases: Activation and cleavage of Gasdermin-D in vitro and during Pyroptosis -- Methods for detecting ASC and ASC speck formation -- Measuring innate immune responses to bacterial viability -- Methods to study cell swelling-induced inflammasome activation -- Detecting release of bacterial dsDNA into the host cytosol using fluorescence microscopy -- Quantitative proteomics of secreted proteins -- Simultaneous detection of cellular viability and interleukin-1β secretion from single cells by ELISpot -- Detection and quantification of MAV aggregation via confocal imaging. .
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| 650 |
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0 |
|a Immunology.
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| 650 |
1 |
4 |
|a Immunology.
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| 700 |
1 |
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|a De Nardo, Dominic.
|e editor.
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| 700 |
1 |
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|a De Nardo, Christine M.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 900 |
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|a Libro descargado a ALEPH en bloque (proveniente de proveedor)
|