Innate Immune Activation : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer New York : Imprint: Humana,
2018.
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| Edición: | 1st ed. 2018. |
| Colección: | Methods in Molecular Biology,
1714 |
| Materias: |
Tabla de Contenidos:
- Emerging concepts in innate immunity
- Bioinformatic Assessment of macrophage activation by the innate immune system
- Generation of Genetic Knockouts in myeloid cell lines using a lentiviral CRISPR/Cas9 system
- Modeling primary human monocytes with the trans-differentiation cell line BLaER1
- Measurement of NF-κB activation in TLR-activated macrophages
- Biochemical isolation of the myddosome from murine macrophages
- Generation of innate immune reporter cell lines using retroviral transduction
- Myddosome Formation by Luminescence-based Mammalian Interactome Mapping (LUMIER)
- Inflammatory caspases: Activation and cleavage of Gasdermin-D in vitro and during Pyroptosis
- Methods for detecting ASC and ASC speck formation
- Measuring innate immune responses to bacterial viability
- Methods to study cell swelling-induced inflammasome activation
- Detecting release of bacterial dsDNA into the host cytosol using fluorescence microscopy
- Quantitative proteomics of secreted proteins
- Simultaneous detection of cellular viability and interleukin-1β secretion from single cells by ELISpot
- Detection and quantification of MAV aggregation via confocal imaging. .