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02676nam a22003135i 4500 |
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20210506150659.0 |
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cr nn 008mamaa |
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101202s2011 xxu| s |||| 0|eng d |
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|a 9781617379673
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| 024 |
7 |
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|a 10.1007/978-1-61737-967-3
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a Heterologous Gene Expression in E.coli :
|b Methods and Protocols /
|c edited by Thomas C. Evans, Jr., Ming-Qun Xu.
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| 250 |
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|a 1st ed. 2011.
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| 260 |
# |
# |
|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2011.
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| 300 |
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|a XI, 310 p. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 705
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| 505 |
0 |
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|a Adjustment of Codon Usage Frequencies by Codon Harmonization Improves Protein Expression and Folding -- SUMO Fusion Technology for Enhanced Protein Expression and Purification in Prokaryotes and Eukaryotes -- Molecular and Chemical Chaperones for Improving the Yields of Soluble Recombinant Proteins -- Genetic Selection of Solubility-Enhanced Proteins Using the Twin-Arginine Translocation System -- Protein Folding Liquid Chromatography -- Site-Specific Protein Labeling by Intein-Mediated Protein Ligation -- Efficient Expression of Human Aromatase (CYP19) in E. coli -- Expression of Recombinant Cytochromes c in E. coli -- Semi-Synthesis of Glycoproteins from E. coli through Native Chemical Ligation -- Expression of Recombinant Proteins with Uniform N-Termini -- Recent Developments in Difficult Protein Expression: A Guide to E. coli Strains, Promoters, and Relevant Host Mutations -- Periplasmic Chaperones Used to Enhance Functional Secretion of Proteins in E. coli -- Engineering Unusual Amino Acids into Peptides Using Lantibiotic Synthetase -- The Targeted Expression of Nucleotide Sugar Transporters to the E. coli Inner Membrane -- Detection of Protein–Protein Interactions in Bacteria by GFP-Fragment Reconstitution -- Enhancing the Solubility of Recombinant Proteins in Escherichia coli by Using Hexahistidine-Tagged Maltose-Binding Protein as a Fusion Partner -- Introducing Predetermined Mutations throughout a Target Gene Using TDEM (Transposon Directed Base-Exchange Mutagenesis) -- Fluorescent Site-Specific Labeling of Escherichia coli Expressed Proteins with Sfp Phosphopantetheinyl Transferase.
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| 650 |
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0 |
|a Human genetics.
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| 650 |
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0 |
|a Proteomics.
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| 650 |
1 |
4 |
|a Human Genetics.
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| 650 |
2 |
4 |
|a Proteomics.
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| 700 |
1 |
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|a Evans, Jr., Thomas C.
|e editor.
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| 700 |
1 |
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|a Xu, Ming-Qun.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
|