Heterologous Gene Expression in E.coli : Methods and Protocols /
| Corporate Author: | |
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| Other Authors: | , |
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2011.
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| Edition: | 1st ed. 2011. |
| Series: | Methods in Molecular Biology,
705 |
| Subjects: |
Table of Contents:
- Adjustment of Codon Usage Frequencies by Codon Harmonization Improves Protein Expression and Folding
- SUMO Fusion Technology for Enhanced Protein Expression and Purification in Prokaryotes and Eukaryotes
- Molecular and Chemical Chaperones for Improving the Yields of Soluble Recombinant Proteins
- Genetic Selection of Solubility-Enhanced Proteins Using the Twin-Arginine Translocation System
- Protein Folding Liquid Chromatography
- Site-Specific Protein Labeling by Intein-Mediated Protein Ligation
- Efficient Expression of Human Aromatase (CYP19) in E. coli
- Expression of Recombinant Cytochromes c in E. coli
- Semi-Synthesis of Glycoproteins from E. coli through Native Chemical Ligation
- Expression of Recombinant Proteins with Uniform N-Termini
- Recent Developments in Difficult Protein Expression: A Guide to E. coli Strains, Promoters, and Relevant Host Mutations
- Periplasmic Chaperones Used to Enhance Functional Secretion of Proteins in E. coli
- Engineering Unusual Amino Acids into Peptides Using Lantibiotic Synthetase
- The Targeted Expression of Nucleotide Sugar Transporters to the E. coli Inner Membrane
- Detection of Protein-Protein Interactions in Bacteria by GFP-Fragment Reconstitution
- Enhancing the Solubility of Recombinant Proteins in Escherichia coli by Using Hexahistidine-Tagged Maltose-Binding Protein as a Fusion Partner
- Introducing Predetermined Mutations throughout a Target Gene Using TDEM (Transposon Directed Base-Exchange Mutagenesis)
- Fluorescent Site-Specific Labeling of Escherichia coli Expressed Proteins with Sfp Phosphopantetheinyl Transferase.