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130705s2013 xxu| s |||| 0|eng d |
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|a 9781627035200
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| 024 |
7 |
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|a 10.1007/978-1-62703-520-0
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a Microglia :
|b Methods and Protocols /
|c edited by Bertrand Joseph, José Luis Venero.
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| 250 |
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|a 1st ed. 2013.
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| 260 |
# |
# |
|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2013.
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| 300 |
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|a XII, 350 p. 65 illus., 50 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 1041
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| 505 |
0 |
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|a A Brief Overview of Multi-Talented Microglia -- Cell Culturing of Human and Murine Microglia Cell Lines -- Microglia Isolation from Adult Mouse Brain -- Preparation of Primary Microglia Cultures from Postnatal Mouse and Rat Brains -- Isolation of Murine Postnatal Brain Microglia for Phenotypic Characterization Using Magnetic Cell Separation Technology -- Isolation and Culture of Adult Human Microglia within Mixed Glial Cultures for Functional Experimentation and High Content Analysis -- Depletion of Microglia from Primary Cellular Cultures -- Lentiviral Transduction of Cultured Microglia -- Microglial Activation: Measurement of Cytokines by Flow Cytometry -- In situ Hybridization of Cytokine mRNA using Alkaline Phosphatase Labelled Oligodeoxynucleotide Probes -- Use of Meso-scale DiscoveryTM to Examine Cytokinecontentin Microglia Cell Supernatant -- Analysis of Microglial Production of Reactive Oxygen and Nitrogen Species -- Quantification of Active Caspase-3 and Active Caspase-8 in Microglia Cells -- Quantification of Microglial Phagocytosis by a Flow Cytometer-based Assay -- Quantification of Microglial Proliferation and Apoptosis by Flow Cytometry -- Fluorescence Imaging of Intracellular Ca2+, Na+ and H+ in Cultured Microglia -- Patch Clamp Protocols to Study Ion Channel Activity in Microglia -- Studying M1 and M2 States in Adult Microglia -- Isolating, Culturing, and Polarizing Primary Human Adult and Fetal Microglia -- Understanding Microglia-neuron Cross-talk: Relevance of the Microglia-neuron Co-cultures -- Preparation of Rodent Primary Cultures for Neuron-Glia, Mixed Glia, Enriched Microglia, and Reconstituted Cultures with Microglia -- Microglia Detection by Enzymatic Histochemistry -- Tomato Lectin Histochemistry for Microglial Visulization -- Immunohistochemical Detection of Microglia -- Intrathecal Infusion of Microglia Cells -- Intracranial Injection of LPS in Rat as Animal Model of Neuroinflammation -- Analyses of Microglia Effector Function Using CX3CR1-GFP Knock In Mice -- In vivo Two-photon Microscopy of Microglia -- Use of Confocal Microscopy in the Study of Microglia in a Brain Metastasis Model.
|
| 650 |
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0 |
|a Neurosciences.
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| 650 |
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0 |
|a Neurobiology.
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| 650 |
1 |
4 |
|a Neurosciences.
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| 650 |
2 |
4 |
|a Neurobiology.
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| 700 |
1 |
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|a Joseph, Bertrand.
|e editor.
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| 700 |
1 |
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|a Venero, José Luis.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
|