Microglia : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2013.
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| Edición: | 1st ed. 2013. |
| Colección: | Methods in Molecular Biology,
1041 |
| Materias: |
Tabla de Contenidos:
- A Brief Overview of Multi-Talented Microglia
- Cell Culturing of Human and Murine Microglia Cell Lines
- Microglia Isolation from Adult Mouse Brain
- Preparation of Primary Microglia Cultures from Postnatal Mouse and Rat Brains
- Isolation of Murine Postnatal Brain Microglia for Phenotypic Characterization Using Magnetic Cell Separation Technology
- Isolation and Culture of Adult Human Microglia within Mixed Glial Cultures for Functional Experimentation and High Content Analysis
- Depletion of Microglia from Primary Cellular Cultures
- Lentiviral Transduction of Cultured Microglia
- Microglial Activation: Measurement of Cytokines by Flow Cytometry
- In situ Hybridization of Cytokine mRNA using Alkaline Phosphatase Labelled Oligodeoxynucleotide Probes
- Use of Meso-scale DiscoveryTM to Examine Cytokinecontentin Microglia Cell Supernatant
- Analysis of Microglial Production of Reactive Oxygen and Nitrogen Species
- Quantification of Active Caspase-3 and Active Caspase-8 in Microglia Cells
- Quantification of Microglial Phagocytosis by a Flow Cytometer-based Assay
- Quantification of Microglial Proliferation and Apoptosis by Flow Cytometry
- Fluorescence Imaging of Intracellular Ca2+, Na+ and H+ in Cultured Microglia
- Patch Clamp Protocols to Study Ion Channel Activity in Microglia
- Studying M1 and M2 States in Adult Microglia
- Isolating, Culturing, and Polarizing Primary Human Adult and Fetal Microglia
- Understanding Microglia-neuron Cross-talk: Relevance of the Microglia-neuron Co-cultures
- Preparation of Rodent Primary Cultures for Neuron-Glia, Mixed Glia, Enriched Microglia, and Reconstituted Cultures with Microglia
- Microglia Detection by Enzymatic Histochemistry
- Tomato Lectin Histochemistry for Microglial Visulization
- Immunohistochemical Detection of Microglia
- Intrathecal Infusion of Microglia Cells
- Intracranial Injection of LPS in Rat as Animal Model of Neuroinflammation
- Analyses of Microglia Effector Function Using CX3CR1-GFP Knock In Mice
- In vivo Two-photon Microscopy of Microglia
- Use of Confocal Microscopy in the Study of Microglia in a Brain Metastasis Model.