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140210s2014 xxu| s |||| 0|eng d |
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|a 9781627039925
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| 024 |
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|a 10.1007/978-1-62703-992-5
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
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|a Monoclonal Antibodies :
|b Methods and Protocols /
|c edited by Vincent Ossipow, Nicolas Fischer.
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| 250 |
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|a 2nd ed. 2014.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2014.
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| 300 |
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|a XIV, 575 p. 108 illus., 27 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
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|a Methods in Molecular Biology,
|v 1131
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| 505 |
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|a Antigen Production for Monoclonal Antibody Generation -- Method for the Generation of Antibodies Specific for Site- and Post-translational Modifications -- Immunization, Hybridoma Generation and Selection for Monoclonal Antibody Production -- Hybridoma Technology for the Generation of Rodent mAbs via Classical Fusion -- Generation of Rabbit Monoclonal Antibodies -- Screening Hybridomas for Cell Surface Antigens by High-throughput Homogenous Assay and Flow Cytometry -- Screening and Subcloning of High Producer Transfectomas using Semi-solid Media and Automated Colony Picker -- Design and Generation of Synthetic Antibody Libraries for Phage Display -- Selection and Screening using Antibody Phage Display Libraries -- Yeast Surface Display for Antibody Isolation: Library Construction, Library Screening and Affinity Maturation -- Human B Cell Immortalization for Monoclonal Antibody Production -- Using Next-generation Sequencing for Discovery of High-frequency Monoclonal Antibodies in the Variable Gene Repertoires from Immunized Mice -- Cloning, Reformatting and Small Scale Expression of Monoclonal Antibody Isolated from Mouse, Rat or Hamster Hybridoma -- Cloning of Recombinant Monoclonal Antibodies from Hybridomas in a Single Mammalian Expression Plasmid -- Monoclonal Antibody Purification by Ceramic Hydroxyapatite Chromatography -- Rapid Purification of Monoclonal Antibodies using Magnetic Microspheres -- Generation of Cell Lines for Monoclonal Antibody Production -- Expression and Purification of Recombinant Antibody Formats and Antibody Fusion Proteins -- Purification of Antibodies and Antibody Fragments using Capture Select Affinity Resins -- Reformatting of scFv antibodies into the scFv—Fc format and their Downstream Purification -- Antibody V and C Domain Sequence, Structure and Interaction Analysis with Special Reference to IMGT® -- Measuring Antibody Affinities as well as the Active Concentration of Antigens Present on a Cell Surface -- Determination of Antibody Structures -- Affinity Maturation of Monoclonal Antibodies by Multi Site-Directed Mutagenesis -- Epitope Mapping with Membrane-bound Synthetic Overlapping Peptides -- Epitope Mapping by Epitope Excision, Hydrogen/Deuterium Exchange and Peptide-Panning Techniques Combined with in silico Analysis -- Fine Epitope Mapping Based on Phage Display and Extensive Mutagenesis of the Target Antigen -- Epitope Mapping with Random Phage Display Library -- Epitope Mapping of Monoclonal and Polyclonal Antibodies using Bacterial Cell Surface Display -- Ion Exchange High Performance Liquid Chromatography (IEX-HPLC) -- Size Exclusion High Performance Liquid Chromatography (SEC-HPLC) -- N-glycosylation Characterization by Liquid Chromatography with Mass Spectrometry -- Fc-Engineering of Antibodies and Antibody Derivatives by Primary Sequence Alteration and their Functional Characterization -- Labeling and use of Monoclonal Antibodies in Immunofluorescence: Protocols for Cytoskeletal and Nuclear Antigens -- Generation and Use of Antibody Fragments for Structural Studies of Proteins Refractory to Crystallization -- Antibody Array Generation and Use.
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| 650 |
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|a Immunology.
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| 650 |
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|a Laboratory medicine.
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| 650 |
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0 |
|a Antibodies.
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| 650 |
1 |
4 |
|a Immunology.
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| 650 |
2 |
4 |
|a Laboratory Medicine.
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| 650 |
2 |
4 |
|a Antibodies.
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| 700 |
1 |
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|a Ossipow, Vincent.
|e editor.
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| 700 |
1 |
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|a Fischer, Nicolas.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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