Monoclonal Antibodies : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2014.
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| Edición: | 2nd ed. 2014. |
| Colección: | Methods in Molecular Biology,
1131 |
| Materias: |
Tabla de Contenidos:
- Antigen Production for Monoclonal Antibody Generation
- Method for the Generation of Antibodies Specific for Site- and Post-translational Modifications
- Immunization, Hybridoma Generation and Selection for Monoclonal Antibody Production
- Hybridoma Technology for the Generation of Rodent mAbs via Classical Fusion
- Generation of Rabbit Monoclonal Antibodies
- Screening Hybridomas for Cell Surface Antigens by High-throughput Homogenous Assay and Flow Cytometry
- Screening and Subcloning of High Producer Transfectomas using Semi-solid Media and Automated Colony Picker
- Design and Generation of Synthetic Antibody Libraries for Phage Display
- Selection and Screening using Antibody Phage Display Libraries
- Yeast Surface Display for Antibody Isolation: Library Construction, Library Screening and Affinity Maturation
- Human B Cell Immortalization for Monoclonal Antibody Production
- Using Next-generation Sequencing for Discovery of High-frequency Monoclonal Antibodies in the Variable Gene Repertoires from Immunized Mice
- Cloning, Reformatting and Small Scale Expression of Monoclonal Antibody Isolated from Mouse, Rat or Hamster Hybridoma
- Cloning of Recombinant Monoclonal Antibodies from Hybridomas in a Single Mammalian Expression Plasmid
- Monoclonal Antibody Purification by Ceramic Hydroxyapatite Chromatography
- Rapid Purification of Monoclonal Antibodies using Magnetic Microspheres
- Generation of Cell Lines for Monoclonal Antibody Production
- Expression and Purification of Recombinant Antibody Formats and Antibody Fusion Proteins
- Purification of Antibodies and Antibody Fragments using Capture Select Affinity Resins
- Reformatting of scFv antibodies into the scFv—Fc format and their Downstream Purification
- Antibody V and C Domain Sequence, Structure and Interaction Analysis with Special Reference to IMGT®
- Measuring Antibody Affinities as well as the Active Concentration of Antigens Present on a Cell Surface
- Determination of Antibody Structures
- Affinity Maturation of Monoclonal Antibodies by Multi Site-Directed Mutagenesis
- Epitope Mapping with Membrane-bound Synthetic Overlapping Peptides
- Epitope Mapping by Epitope Excision, Hydrogen/Deuterium Exchange and Peptide-Panning Techniques Combined with in silico Analysis
- Fine Epitope Mapping Based on Phage Display and Extensive Mutagenesis of the Target Antigen
- Epitope Mapping with Random Phage Display Library
- Epitope Mapping of Monoclonal and Polyclonal Antibodies using Bacterial Cell Surface Display
- Ion Exchange High Performance Liquid Chromatography (IEX-HPLC)
- Size Exclusion High Performance Liquid Chromatography (SEC-HPLC)
- N-glycosylation Characterization by Liquid Chromatography with Mass Spectrometry
- Fc-Engineering of Antibodies and Antibody Derivatives by Primary Sequence Alteration and their Functional Characterization
- Labeling and use of Monoclonal Antibodies in Immunofluorescence: Protocols for Cytoskeletal and Nuclear Antigens
- Generation and Use of Antibody Fragments for Structural Studies of Proteins Refractory to Crystallization
- Antibody Array Generation and Use.