Molecular Toxicology Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Keohavong, Phouthone. (Editor ), Grant, Stephen G. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2014.
Edición:2nd ed. 2014.
Colección:Methods in Molecular Biology, 1105
Materias:
Tabla de Contenidos:
  • Array-Based Immunoassays with Rolling-Circle Amplification Detection
  • Analysis of Protein Changes Using Two Dimensional Difference Gel Electrophoresis
  • Assessment of Pathological and Physiological Changes in Mouse Lung Through Bronchoalveolar Lavage
  • Analysis of Clinical and Biological Samples Using Microsphere-Based Multiplexing Luminex System
  • Detection of DNA Methylation by MeDIP and MBDCap Assays: An Overview of Techniques
  • Screening of DNA Methylation Changes by Methylation-Sensitive Random Amplified Polymorphic DNA-Polymerase Chain Reaction (MS-RAPD-PCR)
  • Strategies for Measurement of Biotransformation Enzyme Gene Expression
  • Genotyping Technologies: Application to Biotransformation Enzyme Genetic Polymorphism Screening
  • TaqMan Fluorogenic Detection System to Analyze Gene Transcription in Autopsy Material
  • 32P-Postlabelling Analysis of DNA Adducts
  • Modification of the 32P-Postlabeling Method to Detect a Single Adduct Species as a Single Spot
  • DNA Isolation and Sample Preparation for Quantitation of Adduct Levels by Accelerator Mass Spectrometry
  • Analysis of DNA Strand Cleavage at Abasic Sites
  • Premature Chromosome Condensation in Human Resting Peripheral Blood Lymphocytes without Mitogen Stimulation for Chromosome Aberration Analysis Using Specific Whole Chromosome DNA Hybridization Probes
  • Mutagen Sensitivity as Measured by Induced Chromatid Breakage as a Marker of Cancer Risk
  • Pulsed-Field Gel Electrophoresis Analysis of Multicellular DNA Double-Strand Break Damage and Repair
  • Detection of Pig-a Mutant Erythrocytes in the Peripheral Blood of Rats and Mice
  • The Blood-Vased Glycophorin A Human In vivo Somatic Mutation Assay
  • Flow Cytometric Quantification of Mutant T Cells with Altered Expression of the T-Cell Receptor: Detecting Somatic Mutants in Humans and Mice
  • Analysis of in Vivo Mutation at the HPRT and TK Genes of Mouse Lymphocytes
  • Quantifying In Vivo Somatic Mutations Using Transgenic Mouse Model Systems
  • The Human T-Cell Cloning Assay: Identifying Genotypes Susceptible to Drug Toxicity and Somatic Mutation
  • Molecular Analysis of HPRT Mutations in Human Lymphocytes
  • Simultaneous Quantification of t(14;18) and HPRT Exon 2/3 Deletions in Human Lymphocytes
  • Mutation Screening of the TP53 Gene by Temporal Temperature Gel Electrophoresis
  • Detection of Point Mutations of K-ras Oncogene and p53 Tumor Suppressor Gene in Sputum Samples
  • ACB-PCR Quantification of Somatic Oncomutations
  • Gel-Based Nonradioactive Single Strand Conformational Polymorphism and Mutation Detection
  • Detection and Characterization of Oncogene Mutations in Preneoplastic and Early Neoplastic Lesion
  • Detection of DNA Double-Strand Breaks and Chromosome Translocations Using Ligation-Mediated PCR and Inverse PCR
  • Quantitative PCR-Based Measurement of Nuclear and Mitochondrial DNA Damage and Repair in Mammalian Cells
  • The Sister Chromatid Exchange (SCE) Assay
  • The Gene Cluster Instability (GCI) Assay for Recombination
  • Measuring Recombination Proficiency in Mouse Embryonic Stem Cells
  • Microsatellite Instability: An Indirect Assay to Detect Defects in the Cellular Mismatch Repair Machinery
  • Unscheduled DNA Synthesis: The Clinical and Functional Assay for Global Genomic Nucleotide Excision Repair
  • An Immunoassay for Measuring Repair of UV Photoproducts
  • Analysis of Double-Strand Break Repair by Non-Homologous DNA End Joining in Cell-Free Extracts from Mammalian Cells
  • Bioenergetic Analysis of Intact Mammalian Cells Using the Seahorse XF42 Extracellular Flux Analyzer and a Luciferase ATP Assay
  • Quantification of Selective Phosphatidylserine Oxidation During Apoptosis
  • Quantitative Method of Measuring Phophatidylserine Externalization During Apoptosis Using Electron Paramagnetic Resonance (EPR) Spectroscopy and Annexin-Conjugated Iron
  • Detection of Programmed Cell Death in Cells Exposed to Genotoxic Agents Using a Caspase Activation Assay.