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130804s2013 xxu| s |||| 0|eng d |
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|a 9781603273695
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| 024 |
7 |
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|a 10.1007/978-1-60327-369-5
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a Lipoproteins and Cardiovascular Disease :
|b Methods and Protocols /
|c edited by Lita A. Freeman.
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| 250 |
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|a 1st ed. 2013.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2013.
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| 300 |
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|a X, 391 p. 50 illus., 23 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 1027
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| 505 |
0 |
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|a Cloning Full-Length Transcripts and Transcript Variants Using 5’ and 3’ RACE -- Monitoring Gene Expression: Quantitative Real-Time RT-PCR -- Microarray Technology: Basic Methodology and Application in Clinical Research for Biomarker Discovery in Vascular Diseases -- Northern Analysis of Gene Expression -- Laser Capture Microdissection for Analysis of Macrophage Gene Expression from Atherosclerotic Lesions -- Sequencing PCR-Amplified DNA in Lipoprotein and Cardiovascular Disease Research -- Introduction to Next-Generation Nucleic Acid Sequencing in Cardiovascular Disease Research -- Strategies for Designing Transgenic DNA Constructs -- Purification of Plasmid and BAC Transgenic DNA Constructs -- Pronuclear Microinjection and Oviduct Transfer Procedures for Transgenic Mouse Production -- Genotyping of Transgenic Animals by Real-Time Quantitative PCR with TaqMan Probes -- Generation of General and Tissue-Specific Gene Knockout Mouse Models -- Adeno-Associated Viruses as Liver-Directed Gene Delivery Vehicles: Focus on Lipoprotein Metabolism -- Modulation of Lipoprotein Metabolism by Antisense Technology: Preclinical Drug Discovery Methodology -- Chromatin Immunoprecipitation -- Measurement of Lecithin:Cholesterol Acyltransferase Activity with the Use of a Peptide-Proteoliposome Substrate -- Native-Native 2D Gel Electrophoresis for HDL Subpopulation Analysis -- Western Blots.
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| 650 |
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0 |
|a Proteins .
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| 650 |
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|a Cardiology.
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| 650 |
1 |
4 |
|a Protein Science.
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| 650 |
2 |
4 |
|a Cardiology.
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| 700 |
1 |
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|a Freeman, Lita A.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
|