Lipoproteins and Cardiovascular Disease : Methods and Protocols /
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| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2013.
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| Edition: | 1st ed. 2013. |
| Series: | Methods in Molecular Biology,
1027 |
| Subjects: |
Table of Contents:
- Cloning Full-Length Transcripts and Transcript Variants Using 5' and 3' RACE
- Monitoring Gene Expression: Quantitative Real-Time RT-PCR
- Microarray Technology: Basic Methodology and Application in Clinical Research for Biomarker Discovery in Vascular Diseases
- Northern Analysis of Gene Expression
- Laser Capture Microdissection for Analysis of Macrophage Gene Expression from Atherosclerotic Lesions
- Sequencing PCR-Amplified DNA in Lipoprotein and Cardiovascular Disease Research
- Introduction to Next-Generation Nucleic Acid Sequencing in Cardiovascular Disease Research
- Strategies for Designing Transgenic DNA Constructs
- Purification of Plasmid and BAC Transgenic DNA Constructs
- Pronuclear Microinjection and Oviduct Transfer Procedures for Transgenic Mouse Production
- Genotyping of Transgenic Animals by Real-Time Quantitative PCR with TaqMan Probes
- Generation of General and Tissue-Specific Gene Knockout Mouse Models
- Adeno-Associated Viruses as Liver-Directed Gene Delivery Vehicles: Focus on Lipoprotein Metabolism
- Modulation of Lipoprotein Metabolism by Antisense Technology: Preclinical Drug Discovery Methodology
- Chromatin Immunoprecipitation
- Measurement of Lecithin:Cholesterol Acyltransferase Activity with the Use of a Peptide-Proteoliposome Substrate
- Native-Native 2D Gel Electrophoresis for HDL Subpopulation Analysis
- Western Blots.