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131024s2014 xxu| s |||| 0|eng d |
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|a 9781627036702
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024 |
7 |
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|a 10.1007/978-1-62703-670-2
|2 doi
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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245 |
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|a Human Retroviruses :
|b Methods and Protocols /
|c edited by Elisa Vicenzi, Guido Poli.
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250 |
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|a 1st ed. 2014.
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260 |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2014.
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300 |
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|a XVIII, 345 p. 62 illus., 38 illus. in color. :
|b online resource.
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336 |
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|a text
|b txt
|2 rdacontent
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|a computer
|b c
|2 rdamedia
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|a online resource
|b cr
|2 rdacarrier
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490 |
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|a Methods in Molecular Biology,
|v 1087
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505 |
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|a Virion Attachment & Entry: HIV gp120 Env Biotinylation, gp120 Env, or Integrin Ligand Binding Assay -- CryoEM Analysis of Capsid Assembly and Structural Changes Upon Interactions with a Host Restriction Factor, TRIM5α -- The Fate of HIV-1 Capsid: A Biochemical Assay for HIV-1 Uncoating -- The Cyclosporin A Washout Assay to Detect HIV-1 Uncoating in Infected Cells -- Imaging HIV-1 Nuclear Pre-Integration Complexes -- HIV-1 Reverse Transcription -- RNase H: Specificity, Mechanisms of Action, and Antiviral Target -- HIV-1 Chromatin, Transcription, and the Regulatory Protein Tat -- HIV-1 Rev Function and RNA Nuclear-Cytoplasmic Export -- HIV-1 Accessory Proteins: Nef -- HIV-1 Accessory Proteins: VpR -- HIV-1 Accessory Proteins: Vpu and Vif -- SIVSM/HIV-2 Vpx Proteins : Function and Uses in the Infection of Primary Myeloid Cells -- Imaging of HIV Assembly and Release -- HIV-1 Isolation from Infected Peripheral Blood Mononuclear Cells -- Determination of HIV-1 Co-Receptor Usage -- The Macrophage and HIV: Basic Concepts and Methodologies -- HIV Infection of Dendritic Cells -- Histocultures (Tissue Explants) in Human Retrovirology -- Single Copy Quantification of HIV-1 in Clinical Samples -- Quantification of Total HIV1-DNA in Peripheral Blood Mononuclear Cells -- HIV-1 Based Lentiviral Vectors -- Quantification of miRNA by Poly(A)-RT-qPCR Arrays and Verification of Target Sites in HIV-1 Using a One-LTR Infectious Molecular Clone -- Investigating Human T Cell Lymphotropic Retrovirus (HTLV) Tax Function with Molecular and Immunophenotypic Techniques -- Proviral Load Determination of HTLV-1 and HTLV-2 in Patients’ Peripheral Blood Mononuclear Cells by Real-Time PCR -- Quantitative Analysis of Human T-Lymphotropic Virus Type 1 (HTLV-1) Gene Expression Using Nucleo-Cytoplasmic Fractionation and Splice Junction-Specific Real Time RT-PCR (qRT-PCR).
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650 |
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|a Immunology.
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650 |
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|a Virology.
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650 |
1 |
4 |
|a Immunology.
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650 |
2 |
4 |
|a Virology.
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700 |
1 |
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|a Vicenzi, Elisa.
|e editor.
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700 |
1 |
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|a Poli, Guido.
|e editor.
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710 |
2 |
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|a SpringerLink (Online service)
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773 |
0 |
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|t Springer eBooks
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