Human Retroviruses : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Vicenzi, Elisa. (Editor ), Poli, Guido. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2014.
Edición:1st ed. 2014.
Colección:Methods in Molecular Biology, 1087
Materias:
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024 7 |a 10.1007/978-1-62703-670-2  |2 doi 
040 |a Sistema de Bibliotecas del Tecnológico de Costa Rica 
245 1 0 |a Human Retroviruses :  |b Methods and Protocols /  |c edited by Elisa Vicenzi, Guido Poli. 
250 |a 1st ed. 2014. 
260 # # |a Totowa, NJ :  |b Humana Press :  |b Imprint: Humana,  |c 2014. 
300 |a XVIII, 345 p. 62 illus., 38 illus. in color. :  |b online resource. 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
338 |a online resource  |b cr  |2 rdacarrier 
490 1 |a Methods in Molecular Biology,  |v 1087 
505 0 |a Virion Attachment & Entry: HIV gp120 Env Biotinylation, gp120 Env, or Integrin Ligand Binding Assay -- CryoEM Analysis of Capsid Assembly and Structural Changes Upon Interactions with a Host Restriction Factor, TRIM5α -- The Fate of HIV-1 Capsid: A Biochemical Assay for HIV-1 Uncoating -- The Cyclosporin A Washout Assay to Detect HIV-1 Uncoating in Infected Cells -- Imaging HIV-1 Nuclear Pre-Integration Complexes -- HIV-1 Reverse Transcription -- RNase H: Specificity, Mechanisms of Action, and Antiviral Target -- HIV-1 Chromatin, Transcription, and the Regulatory Protein Tat -- HIV-1 Rev Function and RNA Nuclear-Cytoplasmic Export -- HIV-1 Accessory Proteins: Nef -- HIV-1 Accessory Proteins: VpR -- HIV-1 Accessory Proteins: Vpu and Vif -- SIVSM/HIV-2 Vpx Proteins : Function and Uses in the Infection of Primary Myeloid Cells -- Imaging of HIV Assembly and Release -- HIV-1 Isolation from Infected Peripheral Blood Mononuclear Cells -- Determination of HIV-1 Co-Receptor Usage -- The Macrophage and HIV: Basic Concepts and Methodologies -- HIV Infection of Dendritic Cells -- Histocultures (Tissue Explants) in Human Retrovirology -- Single Copy Quantification of HIV-1 in Clinical Samples -- Quantification of Total HIV1-DNA in Peripheral Blood Mononuclear Cells -- HIV-1 Based Lentiviral Vectors -- Quantification of miRNA by Poly(A)-RT-qPCR Arrays and Verification of Target Sites in HIV-1 Using a One-LTR Infectious Molecular Clone -- Investigating Human T Cell Lymphotropic Retrovirus (HTLV) Tax Function with Molecular and Immunophenotypic Techniques -- Proviral Load Determination of HTLV-1 and HTLV-2 in Patients’ Peripheral Blood Mononuclear Cells by Real-Time PCR -- Quantitative Analysis of Human T-Lymphotropic  Virus Type 1 (HTLV-1) Gene Expression Using Nucleo-Cytoplasmic Fractionation and Splice Junction-Specific Real Time RT-PCR (qRT-PCR). 
650 0 |a Immunology. 
650 0 |a Virology. 
650 1 4 |a Immunology. 
650 2 4 |a Virology. 
700 1 |a Vicenzi, Elisa.  |e editor. 
700 1 |a Poli, Guido.  |e editor. 
710 2 |a SpringerLink (Online service) 
773 0 |t Springer eBooks