Human Retroviruses : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Vicenzi, Elisa. (Editor ), Poli, Guido. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2014.
Edición:1st ed. 2014.
Colección:Methods in Molecular Biology, 1087
Materias:
Tabla de Contenidos:
  • Virion Attachment & Entry: HIV gp120 Env Biotinylation, gp120 Env, or Integrin Ligand Binding Assay
  • CryoEM Analysis of Capsid Assembly and Structural Changes Upon Interactions with a Host Restriction Factor, TRIM5α
  • The Fate of HIV-1 Capsid: A Biochemical Assay for HIV-1 Uncoating
  • The Cyclosporin A Washout Assay to Detect HIV-1 Uncoating in Infected Cells
  • Imaging HIV-1 Nuclear Pre-Integration Complexes
  • HIV-1 Reverse Transcription
  • RNase H: Specificity, Mechanisms of Action, and Antiviral Target
  • HIV-1 Chromatin, Transcription, and the Regulatory Protein Tat
  • HIV-1 Rev Function and RNA Nuclear-Cytoplasmic Export
  • HIV-1 Accessory Proteins: Nef
  • HIV-1 Accessory Proteins: VpR
  • HIV-1 Accessory Proteins: Vpu and Vif
  • SIVSM/HIV-2 Vpx Proteins : Function and Uses in the Infection of Primary Myeloid Cells
  • Imaging of HIV Assembly and Release
  • HIV-1 Isolation from Infected Peripheral Blood Mononuclear Cells
  • Determination of HIV-1 Co-Receptor Usage
  • The Macrophage and HIV: Basic Concepts and Methodologies
  • HIV Infection of Dendritic Cells
  • Histocultures (Tissue Explants) in Human Retrovirology
  • Single Copy Quantification of HIV-1 in Clinical Samples
  • Quantification of Total HIV1-DNA in Peripheral Blood Mononuclear Cells
  • HIV-1 Based Lentiviral Vectors
  • Quantification of miRNA by Poly(A)-RT-qPCR Arrays and Verification of Target Sites in HIV-1 Using a One-LTR Infectious Molecular Clone
  • Investigating Human T Cell Lymphotropic Retrovirus (HTLV) Tax Function with Molecular and Immunophenotypic Techniques
  • Proviral Load Determination of HTLV-1 and HTLV-2 in Patients’ Peripheral Blood Mononuclear Cells by Real-Time PCR
  • Quantitative Analysis of Human T-Lymphotropic  Virus Type 1 (HTLV-1) Gene Expression Using Nucleo-Cytoplasmic Fractionation and Splice Junction-Specific Real Time RT-PCR (qRT-PCR).