Nanotechnology for Nucleic Acid Delivery : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Ogris, Manfred. (Editor ), Sami, Haider. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2019.
Edición:2nd ed. 2019.
Colección:Methods in Molecular Biology, 1943
Materias:
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024 7 |a 10.1007/978-1-4939-9092-4  |2 doi 
040 |a Sistema de Bibliotecas del Tecnológico de Costa Rica 
245 1 0 |a Nanotechnology for Nucleic Acid Delivery :  |b Methods and Protocols /  |c edited by Manfred Ogris, Haider Sami. 
250 |a 2nd ed. 2019. 
260 # # |a New York, NY :  |b Springer New York :  |b Imprint: Humana,  |c 2019. 
300 |a XV, 406 p. 107 illus., 71 illus. in color. :  |b online resource. 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
338 |a online resource  |b cr  |2 rdacarrier 
490 1 |a Methods in Molecular Biology,  |v 1943 
505 0 |a Sequence-Defined Cationic Lipo-Oligomers Containing Unsaturated Fatty Acids for Transfection -- Synthesis of Bioreducible Polycations with Controlled Topologies -- Histidine-Rich Cationic Cell Penetrating Peptides for Plasmid DNA and siRNA Delivery -- Covalent Fluorophore Labeling of Oligonucleotides and Generation of Other Oligonucleotide Bioconjugates -- Synthetic Oligonucleotides in SPECT/CT In Vivo Imaging: Chemical Modifications, In111 Complex Formation, Incorporation into Drug Delivery Systems -- Synthesis of Polyethylenimine-Based Nanocarriers for Systemic Tumor Targeting of Nucleic Acids -- Cationic Photopolymerized Polydiacetylenic (PDA) Micelles for siRNA Delivery -- Lipids for Nucleic Acid Delivery: Cationic or Neutral Lipoplexes, Synthesis, and Particle Formation -- Preparation, Characterization, and In Vitro Evaluation of Lipidoid-Polymer Hybrid Nanoparticles for siRNA Delivery to the Cytosol -- Layer-By-Layer Assembled Nanoparticles for siRNA Delivery -- Layer-By-Layer Film Engineering for Sequential Gene Delivery -- Surface- and Hydrogel-Mediated Delivery of Nucleic Acid Nanoparticles -- In Situ AFM Analysis Investigating Disassembly of DNA Nanoparticles and Nano-Films -- Lyophilization of Synthetic Gene Carriers -- Firefly Luciferase-Based Reporter Gene Assay for Investigating Nanoparticle-Mediated Nucleic Acid Delivery -- Enhancing Nucleic Acid Delivery with Ultrasound and Microbubbles -- Magnetic and Acoustically Active Microbubbles Loaded with Nucleic Acids for Gene Delivery -- Lactate Dehydrogenase Assay for Assessment of Polycation Cytotoxicity -- Combined Fluorimetric Caspase 3/7 Assay and Bradford Protein Determination for Assessment of Polycation-Mediated Cytotoxicity -- Determination of Polycation-Mediated Perturbation of Mitochondrial Respiration in Intact Cells by High-Resolution Respirometry (Oxygraph-2k, OROBOROS) -- Evaluating the Regulation of Cytokine Levels after siRNA Treatment in Antigen-Specific Target Cell Populations via Intracellular Staining -- Anti-PEG IgM Production via a PEGylated Nano-Carrier System for Nucleic Acid Delivery -- Near-Infrared Optical Imaging of Nucleic Acid Nanocarriers In Vivo -- Flow Cytometry-Based Cell Type-Specific Assessment of Target Regulation by Pulmonary siRNA Delivery -- Microbubbles for Nucleic Acid Delivery in Liver Using Mild Sonoporation -- Lipopeptide Delivery of siRNA to the Central Nervous System. 
650 0 |a Human genetics. 
650 0 |a Nanotechnology. 
650 0 |a Biotechnology. 
650 0 |a Pharmacology. 
650 1 4 |a Human Genetics. 
650 2 4 |a Nanotechnology. 
650 2 4 |a Biotechnology. 
650 2 4 |a Pharmacology/Toxicology. 
700 1 |a Ogris, Manfred.  |e editor. 
700 1 |a Sami, Haider.  |e editor. 
710 2 |a SpringerLink (Online service) 
773 0 |t Springer eBooks 
900 |a Libro descargado a ALEPH en bloque (proveniente de proveedor)