Nanotechnology for Nucleic Acid Delivery : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Ogris, Manfred. (Editor ), Sami, Haider. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2019.
Edición:2nd ed. 2019.
Colección:Methods in Molecular Biology, 1943
Materias:
Tabla de Contenidos:
  • Sequence-Defined Cationic Lipo-Oligomers Containing Unsaturated Fatty Acids for Transfection
  • Synthesis of Bioreducible Polycations with Controlled Topologies
  • Histidine-Rich Cationic Cell Penetrating Peptides for Plasmid DNA and siRNA Delivery
  • Covalent Fluorophore Labeling of Oligonucleotides and Generation of Other Oligonucleotide Bioconjugates
  • Synthetic Oligonucleotides in SPECT/CT In Vivo Imaging: Chemical Modifications, In111 Complex Formation, Incorporation into Drug Delivery Systems
  • Synthesis of Polyethylenimine-Based Nanocarriers for Systemic Tumor Targeting of Nucleic Acids
  • Cationic Photopolymerized Polydiacetylenic (PDA) Micelles for siRNA Delivery
  • Lipids for Nucleic Acid Delivery: Cationic or Neutral Lipoplexes, Synthesis, and Particle Formation
  • Preparation, Characterization, and In Vitro Evaluation of Lipidoid-Polymer Hybrid Nanoparticles for siRNA Delivery to the Cytosol
  • Layer-By-Layer Assembled Nanoparticles for siRNA Delivery
  • Layer-By-Layer Film Engineering for Sequential Gene Delivery
  • Surface- and Hydrogel-Mediated Delivery of Nucleic Acid Nanoparticles
  • In Situ AFM Analysis Investigating Disassembly of DNA Nanoparticles and Nano-Films
  • Lyophilization of Synthetic Gene Carriers
  • Firefly Luciferase-Based Reporter Gene Assay for Investigating Nanoparticle-Mediated Nucleic Acid Delivery
  • Enhancing Nucleic Acid Delivery with Ultrasound and Microbubbles
  • Magnetic and Acoustically Active Microbubbles Loaded with Nucleic Acids for Gene Delivery
  • Lactate Dehydrogenase Assay for Assessment of Polycation Cytotoxicity
  • Combined Fluorimetric Caspase 3/7 Assay and Bradford Protein Determination for Assessment of Polycation-Mediated Cytotoxicity
  • Determination of Polycation-Mediated Perturbation of Mitochondrial Respiration in Intact Cells by High-Resolution Respirometry (Oxygraph-2k, OROBOROS)
  • Evaluating the Regulation of Cytokine Levels after siRNA Treatment in Antigen-Specific Target Cell Populations via Intracellular Staining
  • Anti-PEG IgM Production via a PEGylated Nano-Carrier System for Nucleic Acid Delivery
  • Near-Infrared Optical Imaging of Nucleic Acid Nanocarriers In Vivo
  • Flow Cytometry-Based Cell Type-Specific Assessment of Target Regulation by Pulmonary siRNA Delivery
  • Microbubbles for Nucleic Acid Delivery in Liver Using Mild Sonoporation
  • Lipopeptide Delivery of siRNA to the Central Nervous System.