Protein Secretion : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2010.
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| Edición: | 1st ed. 2010. |
| Colección: | Methods in Molecular Biology,
619 |
| Materias: |
Tabla de Contenidos:
- The Extraordinary Diversity of Bacterial Protein Secretion Mechanisms
- In Vitro and In Vivo Approaches to Studying the Bacterial Signal Peptide Processing
- Membrane Insertion of Small Proteins
- Membrane Protein Insertion in E. coli
- Study of Polytopic Membrane Protein Topological Organization as a Function of Membrane Lipid Composition
- In Vivo Analysis of Protein Translocation to the Escherichia coli Periplasm
- Sorting of Bacterial Lipoproteins to the Outer Membrane by the Lol System
- Purification and Functional Reconstitution of the Bacterial Protein Translocation Pore, the SecYEG Complex
- Reconstitution of the SecY Translocon in Nanodiscs
- In Vitro Assays to Analyze Translocation of the Model Secretory Preprotein Alkaline Phosphatase
- Characterization of Interactions Between Proteins Using Site-Directed Spin Labeling and Electron Paramagnetic Resonance Spectroscopy
- Analysis of Tat Targeting Function and Twin-Arginine Signal Peptide Activity in Escherichia coli
- Site-Specific Cross-Linking of In Vitro Synthesized E. coli Preproteins for Investigating Transmembrane Translocation Pathways
- Tracking the Secretion of Fluorescently Labeled Type III Effectors from Single Bacteria in Real Time
- Comparative Analysis of Cytoplasmic Membrane Proteomes of Escherichia coli Using 2D Blue Native/SDS-PAGE
- Using Hidden Markov Models to Discover New Protein Transport Machines
- Bioinformatics Predictions of Localization and Targeting
- The Chloroplast Protein Import Machinery: A Review
- Measurement of the Energetics of Protein Transport Across the Chloroplast Thylakoid Membrane
- In Vitro Dissection of Protein Translocation into the Mammalian Endoplasmic Reticulum
- In Vitro Reconstitution of the Selection, Ubiquitination, and Membrane Extraction of a Polytopic ERAD Substrate
- Studying the ArfGAP-Dependent Conformational Changes in SNAREs
- Studying Endoplasmic Reticulum Function In Vitro Using siRNA
- High-Quality Immunofluorescence of Cultured Cells
- Trapping Oxidative Folding Intermediates During Translocation to the Intermembrane Space of Mitochondria: In Vivo and In Vitro Studies
- Native Techniques for Analysis of Mitochondrial Protein Import.