NADPH Oxidases : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Knaus, Ulla G. (Editor ), Leto, Thomas L. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2019.
Edición:1st ed. 2019.
Colección:Methods in Molecular Biology, 1982
Materias:
Tabla de Contenidos:
  • Intersecting Stories of the Phagocyte NADPH Oxidase and Chronic Granulomatous Disease
  • Mammalian NADPH Oxidases
  • Enhanced Immunoaffinity Purification of Human Neutrophil Flavocytochrome B for Structure Determination by Electron Microscopy
  • Purification and Characterization of DUOX Peroxidase Homology Domains (PHDs)
  • A Close-Up View of the Impact of Arachidonic Acid on the Phagocyte NADPH Oxidase
  • NOX5 Cell-Free Assay for the High-Throughput Screening of Small Molecules
  • Spectroscopy of NOX Protein Family Members
  • Soluble Regulatory Proteins for Activation of NOX Family NADPH Oxidases
  • Insights into the NOX NADPH Oxidases Using Heterologous Whole Cell Assays
  • The X-CGD PLB-985 Cell Model for NOX2 Structure-Function Analysis
  • Functional Characterization of DUOX Enzymes in Reconstituted Cell Models
  • Guidelines for the Detection of NADPH Oxidases by Immunoblot and RT-qPCR
  • Methods for Detection of NOX-Derived Superoxide Radical Anion and Hydrogen Peroxide in Cells
  • HPLC-Based Monitoring of Oxidation of Hydroethidine for Detection of NADPH Oxidase-Derived Superoxide Radical Anion
  • Visualization of Intracellular Hydrogen Peroxide with the Genetically-Encoded Fluorescent Probe HyPer in NIH-3T3 Cells
  • Imaging Intracellular H2O2 with the Genetically-Encoded PerFRET and OxyFRET Probes
  • Quantitative Imaging of Endogenous and Exogenous H2O2 Gradients in Live Zebrafish Larvae
  • Kinetic Analysis of Phagosomal ROS Generation
  • Imaging Intestinal ROS in Homeostatic Conditions Using L-012
  • Hydro-Cy3-Mediated Detection of Reactive Oxygen Species In Vitro and In Vivo
  • Phosphorylation of Gp91phox/NOX2 in Human Neutrophils
  • The Molecular Regulation and Functional Roles of NOX5
  • Using Synthetic Peptides for Exploring Protein-Protein Interactions in the Assembly of the NADPH Oxidase Complex
  • Rational Design and Delivery of NOX-Inhibitory Peptides
  • High-Throughput Screening of NOX Inhibitors
  • Protein-Protein Interaction Assay to Analyze NOX4/p22phox Heterodimerization
  • Isolation of Redox Active Endosomes (Redoxosomes) and Assessment of NOX Activity
  • Model Systems to Investigate NOX-Dependent Cell Migration and Invasiveness
  • NADPH Oxidases and Aging Models of Lung Fibrosis
  • Proteomic Methods to Evaluate NOX-Mediated Redox Signaling
  • Neutrophil Extracellular Traps
  • Chronic Granulomatous Disease
  • Diagnostic Testing for Chronic Granulomatous Disease
  • Gastrointestinal Complications in Chronic Granulomatous Disease
  • Ex Vivo Models of Chronic Granulomatous Disease
  • Gene Editing in Chronic Granulomatous Disease
  • DUOX Defects and Their Roles in Congenital Hypothyroidism
  • NAPDH Oxidases in Inflammatory Bowel Disease.