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111011s2012 xxu| s |||| 0|eng d |
| 020 |
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|a 9781617793523
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| 024 |
7 |
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|a 10.1007/978-1-61779-352-3
|2 doi
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| 040 |
|
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a Protein Expression in Mammalian Cells :
|b Methods and Protocols /
|c edited by James L Hartley.
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| 250 |
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|a 1st ed. 2012.
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| 260 |
# |
# |
|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2012.
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| 300 |
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|a XI, 276 p. 48 illus., 9 illus. in color. :
|b online resource.
|
| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 801
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| 505 |
0 |
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|a Why Proteins in Mammalian Cells? -- Large Scale Transfection of Mammalian Cells -- Selection of High Expressing Mammalian Cells by Surface Display of Reporters -- Expression of a Secreted Protein in Mammalian Cells Using Baculovirus Particles -- Transfection of Difficult-to-Transfect Primary Mammalian Cells -- Stable Protein Expression in Mammalian Cells Using Baculoviruses -- Using Matrix Attachment Regions to Improve Recombinant Protein Production -- Controlling Apoptosis to Optimize Yields of Proteins from Mammalian Cells -- Post-Transcriptional Regulatory Elements for Enhancing Transient Gene Expression Levels in Mammalian Cells -- Converting Monoclonal Antibodies into Fab Fragments for Transient Expression in Mammalian Cells -- Generation of High-Expressing Cells by Methotrexate Amplification of Destabilized Dihydrofolate Reductase Selection Marker -- Tools for Co-Producing Multiple Proteins in Mammalian Cells -- Identification and Characterization of Protein Glycosylation Using Specific Endo- and Exoglycosidases -- Strategies for the Efficient Transfection of CHO-Cells with Plasmid DNA -- Methods for Constructing Clones for Protein Expression in Mammalian Cells -- Optimizing Transient Recombinant Protein Expression in Mammalian Cells.
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| 650 |
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0 |
|a Proteins .
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| 650 |
1 |
4 |
|a Protein Science.
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| 700 |
1 |
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|a Hartley, James L.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 900 |
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|a Libro descargado a ALEPH en bloque (proveniente de proveedor)
|