Protein Expression in Mammalian Cells : Methods and Protocols /
| Corporate Author: | |
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| Other Authors: | |
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2012.
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| Edition: | 1st ed. 2012. |
| Series: | Methods in Molecular Biology,
801 |
| Subjects: |
Table of Contents:
- Why Proteins in Mammalian Cells?
- Large Scale Transfection of Mammalian Cells
- Selection of High Expressing Mammalian Cells by Surface Display of Reporters
- Expression of a Secreted Protein in Mammalian Cells Using Baculovirus Particles
- Transfection of Difficult-to-Transfect Primary Mammalian Cells
- Stable Protein Expression in Mammalian Cells Using Baculoviruses
- Using Matrix Attachment Regions to Improve Recombinant Protein Production
- Controlling Apoptosis to Optimize Yields of Proteins from Mammalian Cells
- Post-Transcriptional Regulatory Elements for Enhancing Transient Gene Expression Levels in Mammalian Cells
- Converting Monoclonal Antibodies into Fab Fragments for Transient Expression in Mammalian Cells
- Generation of High-Expressing Cells by Methotrexate Amplification of Destabilized Dihydrofolate Reductase Selection Marker
- Tools for Co-Producing Multiple Proteins in Mammalian Cells
- Identification and Characterization of Protein Glycosylation Using Specific Endo- and Exoglycosidases
- Strategies for the Efficient Transfection of CHO-Cells with Plasmid DNA
- Methods for Constructing Clones for Protein Expression in Mammalian Cells
- Optimizing Transient Recombinant Protein Expression in Mammalian Cells.