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03732nam a22002895i 4500 |
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000288232 |
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20210910091214.0 |
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cr nn 008mamaa |
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160909s2016 xxu| s |||| 0|eng d |
| 020 |
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|a 9781493937561
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| 024 |
7 |
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|a 10.1007/978-1-4939-3756-1
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Proteostasis :
|b Methods and Protocols /
|c edited by Rune Matthiesen.
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| 250 |
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|a 1st ed. 2016.
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| 260 |
# |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2016.
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| 300 |
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|a XVI, 488 p. 94 illus., 60 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
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|a Methods in Molecular Biology,
|v 1449
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| 505 |
0 |
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|a UPS Activation in the Battle against Aging and Aggregation-Related Diseases: An Extended Review -- Review And Literature Mining On Proteostasis Factors’ And Cancer -- Combining Zebrafish and Mouse Models to Test the Function of Deubiquitinating Enzyme (Dubs) Genes in Development: Role Of Usp45 in the Retina -- Immunodepletion and Immunopurification as Approaches for CSN Research -- Studying Protein Ubiquitylation in Yeast -- Strategies to Detect Endogenous Ubiquitination of a Target Mammalian Protein -- In Vitro Ubiquitination – Self-Ubiquitination, Chain Formation, and Substrate Ubiquitination Assays -- Isolation of the Ubiquitin-Proteome From Tumour Cell Lines and Primary Cells Using Tubes -- Tubes-Mass Spectrometry for Identification and Analysis of the Ubiquitin-Proteome -- Isolation of Ubiquitinated Proteins to High Purity from In Vivo Samples -- Method for the Purification of Endogenous Unanchored Polyubiquitin Chains -- Fluorescent Tools for In Vivo Studies on the Ubiquitin-Proteasome System -- Bimolecular Fluorescence Complementation to Assay the Interactions of Ubiquitylation Enzymes in Living Yeast Cells -- Monitoring Ubiquitin-Coated Bacteria Via Confocal Microscopy -- Detection and Analysis of Cell Cycle-Associated Apc/C-Mediated Cellular Ubiquitylation In Vitro and In Vivo -- Detection and Analysis Of Sumoylation Substrates In Vitro and In Vivo. - Detection of Protein-Protein Interactions and Post-Translational Modifications using the Proximity Ligation Assay: Application to the Study of the Sumo Pathway -- Dissecting of Sumo Dynamics by Mass Spectrometry -- Isolation of Lysosomes from Mammalian Tissues and Cultured Cells -- Analysis Of Relevant Parameters For Autophagic Flux Using Hela Cells Expressing Egfp-Lc3 -- Analysis of Protein Oligomeric Species by Sucrose Gradients -- Analysis of Protein Oligomerization by Electrophoresis.-Blot-Ms of Carbonylated Proteins: A Tool to Identify Oxidized Proteins -- Quantitation of Protein Translation Rate In Vivo with Bioorthogonal Click-Chemistry -- A Simple Protocol for High Efficiency Protein Isolation after RNA Isolation from Mouse Thyroid and Other Very Small Tissue Samples -- Monitoring Target Engagement Of Deubiquitylating Enzymes Using Activity Probes: Past, Present, and Future -- Activity Based Profiling of Deubiquitylating Enzymes and Inhibitors in Animal Tissues -- High-Throughput Sirna Screening Applied to the Ubiquitin-Proteasome System -- High-Throughput Yeast-Based Reporter Assay to Identify Compounds with Anti-Inflammatory Potential -- Using Alphascreen ® to Identify Small Molecule Inhibitors Targeting a Conserved Host-Pathogen Interaction -- Global MS-Based Proteomics Drug Profiling.
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| 650 |
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|a Proteins .
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| 650 |
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4 |
|a Protein Science.
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| 700 |
1 |
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|a Matthiesen, Rune.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 900 |
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|a Libro descargado a ALEPH en bloque (proveniente de proveedor)
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