Proteostasis : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Matthiesen, Rune. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2016.
Edición:1st ed. 2016.
Colección:Methods in Molecular Biology, 1449
Materias:
Tabla de Contenidos:
  • UPS Activation in the Battle against Aging and Aggregation-Related Diseases: An Extended Review
  • Review And Literature Mining On Proteostasis Factors’ And Cancer
  • Combining Zebrafish and Mouse Models to Test the Function of Deubiquitinating Enzyme (Dubs) Genes in Development: Role Of Usp45 in the Retina
  • Immunodepletion and Immunopurification as Approaches for CSN Research
  • Studying Protein Ubiquitylation in Yeast
  • Strategies to Detect Endogenous Ubiquitination of a Target Mammalian Protein
  • In Vitro Ubiquitination – Self-Ubiquitination, Chain Formation, and Substrate Ubiquitination Assays
  • Isolation of the Ubiquitin-Proteome From Tumour Cell Lines and Primary Cells Using Tubes
  • Tubes-Mass Spectrometry for Identification and Analysis of the Ubiquitin-Proteome
  • Isolation of Ubiquitinated Proteins to High Purity from In Vivo Samples
  • Method for the Purification of Endogenous Unanchored Polyubiquitin Chains
  • Fluorescent Tools for In Vivo Studies on the Ubiquitin-Proteasome System
  • Bimolecular Fluorescence Complementation to Assay the Interactions of Ubiquitylation Enzymes in Living Yeast Cells
  • Monitoring Ubiquitin-Coated Bacteria Via Confocal Microscopy
  • Detection and Analysis of Cell Cycle-Associated Apc/C-Mediated Cellular Ubiquitylation In Vitro and In Vivo
  • Detection and Analysis Of Sumoylation Substrates In Vitro and In Vivo. - Detection of Protein-Protein Interactions and Post-Translational Modifications using the Proximity Ligation Assay: Application to the Study of the Sumo Pathway
  • Dissecting of Sumo Dynamics by Mass Spectrometry
  • Isolation of Lysosomes from Mammalian Tissues and Cultured Cells
  • Analysis Of Relevant Parameters For Autophagic Flux Using Hela Cells Expressing Egfp-Lc3
  • Analysis of Protein Oligomeric Species by Sucrose Gradients
  • Analysis of Protein Oligomerization by Electrophoresis.-Blot-Ms of Carbonylated Proteins: A Tool to Identify Oxidized Proteins
  • Quantitation of Protein Translation Rate In Vivo with Bioorthogonal Click-Chemistry
  • A Simple Protocol for High Efficiency Protein Isolation after RNA Isolation from Mouse Thyroid and Other Very Small Tissue Samples
  • Monitoring Target Engagement Of Deubiquitylating Enzymes Using Activity Probes: Past, Present, and Future
  • Activity Based Profiling of Deubiquitylating Enzymes and Inhibitors in Animal Tissues
  • High-Throughput Sirna Screening Applied to the Ubiquitin-Proteasome System
  • High-Throughput Yeast-Based Reporter Assay to Identify Compounds with Anti-Inflammatory Potential
  • Using Alphascreen ® to Identify Small Molecule Inhibitors Targeting a Conserved Host-Pathogen Interaction
  • Global MS-Based Proteomics Drug Profiling.