Proteostasis : Methods and Protocols /
Autor Corporativo: | |
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Otros Autores: | |
Formato: | eBook |
Lenguaje: | English |
Publicado: |
New York, NY :
Springer New York : Imprint: Humana,
2016.
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Edición: | 1st ed. 2016. |
Colección: | Methods in Molecular Biology,
1449 |
Materias: |
Tabla de Contenidos:
- UPS Activation in the Battle against Aging and Aggregation-Related Diseases: An Extended Review
- Review And Literature Mining On Proteostasis Factors’ And Cancer
- Combining Zebrafish and Mouse Models to Test the Function of Deubiquitinating Enzyme (Dubs) Genes in Development: Role Of Usp45 in the Retina
- Immunodepletion and Immunopurification as Approaches for CSN Research
- Studying Protein Ubiquitylation in Yeast
- Strategies to Detect Endogenous Ubiquitination of a Target Mammalian Protein
- In Vitro Ubiquitination – Self-Ubiquitination, Chain Formation, and Substrate Ubiquitination Assays
- Isolation of the Ubiquitin-Proteome From Tumour Cell Lines and Primary Cells Using Tubes
- Tubes-Mass Spectrometry for Identification and Analysis of the Ubiquitin-Proteome
- Isolation of Ubiquitinated Proteins to High Purity from In Vivo Samples
- Method for the Purification of Endogenous Unanchored Polyubiquitin Chains
- Fluorescent Tools for In Vivo Studies on the Ubiquitin-Proteasome System
- Bimolecular Fluorescence Complementation to Assay the Interactions of Ubiquitylation Enzymes in Living Yeast Cells
- Monitoring Ubiquitin-Coated Bacteria Via Confocal Microscopy
- Detection and Analysis of Cell Cycle-Associated Apc/C-Mediated Cellular Ubiquitylation In Vitro and In Vivo
- Detection and Analysis Of Sumoylation Substrates In Vitro and In Vivo. - Detection of Protein-Protein Interactions and Post-Translational Modifications using the Proximity Ligation Assay: Application to the Study of the Sumo Pathway
- Dissecting of Sumo Dynamics by Mass Spectrometry
- Isolation of Lysosomes from Mammalian Tissues and Cultured Cells
- Analysis Of Relevant Parameters For Autophagic Flux Using Hela Cells Expressing Egfp-Lc3
- Analysis of Protein Oligomeric Species by Sucrose Gradients
- Analysis of Protein Oligomerization by Electrophoresis.-Blot-Ms of Carbonylated Proteins: A Tool to Identify Oxidized Proteins
- Quantitation of Protein Translation Rate In Vivo with Bioorthogonal Click-Chemistry
- A Simple Protocol for High Efficiency Protein Isolation after RNA Isolation from Mouse Thyroid and Other Very Small Tissue Samples
- Monitoring Target Engagement Of Deubiquitylating Enzymes Using Activity Probes: Past, Present, and Future
- Activity Based Profiling of Deubiquitylating Enzymes and Inhibitors in Animal Tissues
- High-Throughput Sirna Screening Applied to the Ubiquitin-Proteasome System
- High-Throughput Yeast-Based Reporter Assay to Identify Compounds with Anti-Inflammatory Potential
- Using Alphascreen ® to Identify Small Molecule Inhibitors Targeting a Conserved Host-Pathogen Interaction
- Global MS-Based Proteomics Drug Profiling.