RNA-Protein Complexes and Interactions : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Lin, Ren-Jang. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2016.
Edición:1st ed. 2016.
Colección:Methods in Molecular Biology, 1421
Materias:
LEADER 02948nam a22002895i 4500
001 000288279
005 20210910132644.0
007 cr nn 008mamaa
008 160310s2016 xxu| s |||| 0|eng d
020 |a 9781493935918 
024 7 |a 10.1007/978-1-4939-3591-8  |2 doi 
040 |a Sistema de Bibliotecas del Tecnológico de Costa Rica 
245 1 0 |a RNA-Protein Complexes and Interactions :  |b Methods and Protocols /  |c edited by Ren-Jang Lin. 
250 |a 1st ed. 2016. 
260 # # |a New York, NY :  |b Springer New York :  |b Imprint: Humana,  |c 2016. 
300 |a XIII, 288 p. 49 illus., 24 illus. in color. :  |b online resource. 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
338 |a online resource  |b cr  |2 rdacarrier 
490 1 |a Methods in Molecular Biology,  |v 1421 
505 0 |a Characterization of RNA-Protein Interactions: Lessons from Two RNA-Binding Proteins, SRSF1 and SRSF2 -- Identification of mRNA-Interacting Factors by MS2-TRAP (MS2-Tagged RNA Affinity Purification) -- Biotin-Streptavidin Affinity Purification of RNA-Protein Complexes Assembled In Vitro -- Detecting RNA-Protein Interaction Using End-Labeled Biotinylated RNA Oligonucleotides and Immunoblotting -- Purification of RNA-Protein Splicing Complexes Using a Tagged Protein from In Vitro Splicing Reaction Mixture -- Loading of Argonaute Protein with Small Duplex RNA in Cellular Extracts -- In Vitro Analysis of Ribonucleoprotein Complex Remodeling and Disassembly -- Single-Turnover Kinetics of Methyl Transfer to tRNA by Methyltransferases -- Purification and Functional Reconstitution of Box H/ACA Ribonucleoprotein Particles -- Northwestern Blot Analysis: Detecting RNA-Protein Interaction After Gel Separation of Protein Mixture -- Measuring mRNA Translation by Polysome Profiling -- Genome-Wide Profiling of RNA-Protein Interactions Using CLIP-Seq -- Identification of Endogenous mRNA-Binding Proteins in Yeast Using Crosslinking and PolyA Enrichment -- Ribo-Proteomics Approach to Profile RNA-Protein and Protein-Protein Interaction Networks -- Detection of Protein-Protein Interaction Within an RNA-Protein Complex Via Unnatural-Amino-Acid-Mediated Photochemical Crosslinking -- Evolution of Cell-Type-Specific RNA Aptamers Via Live Cell-Based SELEX -- mCarts: Genome-Wide Prediction of Clustered Sequence Motifs as Binding Sites for RNA Binding Proteins -- Design of RNA-Binding Proteins: Manipulate Alternative Splicing in Human Cells with Artificial Splicing Factors -- Informational Suppression to Probe RNA:RNA Interactions in the Context of Ribonucleoproteins: U1 and 5’ Splice-Site Base-Pairing -- Analysis of Alternative Pre-RNA Splicing in the Mouse Retina Using a Fluorescent Reporter. 
650 0 |a Proteins . 
650 1 4 |a Protein Science. 
700 1 |a Lin, Ren-Jang.  |e editor. 
710 2 |a SpringerLink (Online service) 
773 0 |t Springer eBooks 
900 |a Libro descargado a ALEPH en bloque (proveniente de proveedor)