RNA-Protein Complexes and Interactions : Methods and Protocols /
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer New York : Imprint: Humana,
2016.
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| Edición: | 1st ed. 2016. |
| Colección: | Methods in Molecular Biology,
1421 |
| Materias: |
Tabla de Contenidos:
- Characterization of RNA-Protein Interactions: Lessons from Two RNA-Binding Proteins, SRSF1 and SRSF2
- Identification of mRNA-Interacting Factors by MS2-TRAP (MS2-Tagged RNA Affinity Purification)
- Biotin-Streptavidin Affinity Purification of RNA-Protein Complexes Assembled In Vitro
- Detecting RNA-Protein Interaction Using End-Labeled Biotinylated RNA Oligonucleotides and Immunoblotting
- Purification of RNA-Protein Splicing Complexes Using a Tagged Protein from In Vitro Splicing Reaction Mixture
- Loading of Argonaute Protein with Small Duplex RNA in Cellular Extracts
- In Vitro Analysis of Ribonucleoprotein Complex Remodeling and Disassembly
- Single-Turnover Kinetics of Methyl Transfer to tRNA by Methyltransferases
- Purification and Functional Reconstitution of Box H/ACA Ribonucleoprotein Particles
- Northwestern Blot Analysis: Detecting RNA-Protein Interaction After Gel Separation of Protein Mixture
- Measuring mRNA Translation by Polysome Profiling
- Genome-Wide Profiling of RNA-Protein Interactions Using CLIP-Seq
- Identification of Endogenous mRNA-Binding Proteins in Yeast Using Crosslinking and PolyA Enrichment
- Ribo-Proteomics Approach to Profile RNA-Protein and Protein-Protein Interaction Networks
- Detection of Protein-Protein Interaction Within an RNA-Protein Complex Via Unnatural-Amino-Acid-Mediated Photochemical Crosslinking
- Evolution of Cell-Type-Specific RNA Aptamers Via Live Cell-Based SELEX
- mCarts: Genome-Wide Prediction of Clustered Sequence Motifs as Binding Sites for RNA Binding Proteins
- Design of RNA-Binding Proteins: Manipulate Alternative Splicing in Human Cells with Artificial Splicing Factors
- Informational Suppression to Probe RNA:RNA Interactions in the Context of Ribonucleoproteins: U1 and 5’ Splice-Site Base-Pairing
- Analysis of Alternative Pre-RNA Splicing in the Mouse Retina Using a Fluorescent Reporter.