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160717s2016 xxu| s |||| 0|eng d |
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|a 9781493937592
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| 024 |
7 |
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|a 10.1007/978-1-4939-3759-2
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Plant Proteostasis :
|b Methods and Protocols /
|c edited by L. Maria Lois, Rune Matthiesen.
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| 250 |
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|a 1st ed. 2016.
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| 260 |
# |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2016.
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| 300 |
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|a XI, 307 p. 73 illus., 40 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 1450
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| 505 |
0 |
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|a Approaches to Determine Protein Ubiquitination Residue Types -- Immunoprecipitation of Cullin Ring Ligases (CRLs) in Arabidopsis Thaliana Seedlings -- Radioligand Binding Assays for Determining Dissociation Constants of Phytohormone Receptors -- Measuring the Enzyme Activity of Arabidopsis Deubiquitylating Enzymes -- Fluorescent Reporters for Ubiquitin-Dependent Proteolysis in Plants -- Generation of Artificial N-end Rule Substrate Proteins In Vivo and In Vitro -- Peptide Arrays for Binding Studies of E3 Ubiquitin Ligases -- SUMO Chain Formation by Plants Enzymes -- Kinetic Analysis of Plant SUMO Conjugation Machinery -- Expression, Purification, and Enzymatic Analysis Of Plant SUMO Proteases -- Quantitative Analysis of Subcellular Distribution of the SUMO Conjugation System by Confocal Microscopy Imaging -- Biochemical Analysis of Autophagy in Algae and Plants by Monitoring the Electrophoretic Mobility of ATG8 -- Detection of Autophagy in Plants by Fluorescence Microscopy -- Protocols for Studying Protein Stability in a Arabidopsis Protoplast Transient Expression System -- Detection and Quantification of Protein Aggregates in Plants -- Determination of Protein Carbonylation and Proteasome Activity in Seeds -- Isobaric Tag for Relative and Absolute Quantitation (iTRAQ)-based Protein Profiling in Plants.-Use of a Phosphatidylinositol phosphate Affinity Chromatography (PIP - chromatography) for the Isolation of Proteins Involved in Protein Quality Control and Proteostasis Mechanisms in Plants -- In vivo Radiolabeling of Arabidopsis Leaves and Separation of Thylakoid Membrane Complexes by Blue Native PAGE -- Normalized Quantitative Western Blotting Based on Standardized Fluorescent Labeling -- Sequence Search and Comparative Genomic Analysis of SUMO-Activating Enzymes using CoGe -- Studying Evolutionary Dynamics of Gene Families Encoding SUMO-Activating Enzymes with SeaView and ProtTest -- Bioinformatics Tools for Exploring the SUMO Gene Network. .
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| 650 |
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|a Plant science.
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| 650 |
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|a Botany.
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| 650 |
1 |
4 |
|a Plant Sciences.
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| 700 |
1 |
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|a Lois, L. Maria.
|e editor.
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| 700 |
1 |
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|a Matthiesen, Rune.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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