Plant Proteostasis : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer New York : Imprint: Humana,
2016.
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| Edición: | 1st ed. 2016. |
| Colección: | Methods in Molecular Biology,
1450 |
| Materias: |
Tabla de Contenidos:
- Approaches to Determine Protein Ubiquitination Residue Types
- Immunoprecipitation of Cullin Ring Ligases (CRLs) in Arabidopsis Thaliana Seedlings
- Radioligand Binding Assays for Determining Dissociation Constants of Phytohormone Receptors
- Measuring the Enzyme Activity of Arabidopsis Deubiquitylating Enzymes
- Fluorescent Reporters for Ubiquitin-Dependent Proteolysis in Plants
- Generation of Artificial N-end Rule Substrate Proteins In Vivo and In Vitro
- Peptide Arrays for Binding Studies of E3 Ubiquitin Ligases
- SUMO Chain Formation by Plants Enzymes
- Kinetic Analysis of Plant SUMO Conjugation Machinery
- Expression, Purification, and Enzymatic Analysis Of Plant SUMO Proteases
- Quantitative Analysis of Subcellular Distribution of the SUMO Conjugation System by Confocal Microscopy Imaging
- Biochemical Analysis of Autophagy in Algae and Plants by Monitoring the Electrophoretic Mobility of ATG8
- Detection of Autophagy in Plants by Fluorescence Microscopy
- Protocols for Studying Protein Stability in a Arabidopsis Protoplast Transient Expression System
- Detection and Quantification of Protein Aggregates in Plants
- Determination of Protein Carbonylation and Proteasome Activity in Seeds
- Isobaric Tag for Relative and Absolute Quantitation (iTRAQ)-based Protein Profiling in Plants.-Use of a Phosphatidylinositol phosphate Affinity Chromatography (PIP - chromatography) for the Isolation of Proteins Involved in Protein Quality Control and Proteostasis Mechanisms in Plants
- In vivo Radiolabeling of Arabidopsis Leaves and Separation of Thylakoid Membrane Complexes by Blue Native PAGE
- Normalized Quantitative Western Blotting Based on Standardized Fluorescent Labeling
- Sequence Search and Comparative Genomic Analysis of SUMO-Activating Enzymes using CoGe
- Studying Evolutionary Dynamics of Gene Families Encoding SUMO-Activating Enzymes with SeaView and ProtTest
- Bioinformatics Tools for Exploring the SUMO Gene Network. .