Neurodegeneration : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Manfredi, Giovanni. (Editor ), Kawamata, Hibiki. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2011.
Edición:1st ed. 2011.
Colección:Methods in Molecular Biology, 793
Materias:
Tabla de Contenidos:
  • Introduction to Neurodegenerative Diseases and Related Techniques
  • In vivo and In vitro Determination of Cell Death Markers in Neurons
  • Degenerating Processes Identified by Electron Microscopic Immunocytochemical Methods
  • Isolation and Culture of Neurons and Astrocytes from the Mouse Brain Cortex
  • Isolation and Culture of Postnatal Spinal Motoneurons
  • Converting Human Pluripotent Stem Cells to Neural Tissue and Neurons to Model Neurodegeneration
  • Neural Differentiation of Induced Pluripotent Stem Cells
  • Developing Yeast Models of Human Neurodegenerative Disorders
  • Modeling Dopamine Neuron Degeneration in C. elegans
  • Role of the Proteasome in Fly Models of Neurodegeneration
  • Modeling Neurodegenerative Diseases in Zebrafish Embryos
  • Practical Considerations of Genetic Rodent Models for Neurodegenerative Diseases
  • Modeling Focal Cerebral Ischemia In vivo
  • Pharmacological Models of Parkison's Disease in Rodents
  • Behavioral Phenotyping of Mouse Models of Neurodegeneration
  • Characterization of Amyloid Deposits in Neurodegenerative Diseases
  • Detection of Protein Aggregates in Neurodegenerative Diseases
  • Assessment of Proteasome Impairment and Accumulation/Aggregation of Ubiquitinated Proteins in Neuronal Cultures
  • Measurements of Threshold of Mitochondrial Transition Pore Opening in Intact and Permeabilized Cells by Flash Photolysis of Caged Calcium
  • Isolation and Functional Assessment of Mitochondria from Small Amounts of Mouse Brain Tissue
  • Monitoring Mitophagy in Neuronal Cell Cultures
  • Analysis of Vesicular Trafficking in Primary Neurons by Live Imaging
  • Surface Trafficking of Sodium Channels in Cells and in Hippocampal Slices
  • Imaging Presynaptic Exocytosis in Corticostriatal Slices
  • Microscopic Imaging of Intracellular Calcium in Live Cells using Lifetime based Ratiometric Measurements of Oregon Green BAPTA-1
  • Electrophysiological Characterization of Neuromuscular Synaptic Dysfunction in Mice
  • Determination of Neurotransmitter Levels in Models of Parkinson’s Disease by HPLC-ECD
  • Designing, Performing, and Interpreting a Microarray-based Gene Expression Study
  • Adeno-associated Viral Gene Transfer for Neurodegenerative Diseases
  • Specific Gene Silencing using RNAi in Cell Culture
  • Stem Cell Transplantation for Spinal Cord Neurodegeneration
  • Evaluation of Histone Deacetylase Inhibitors as Therapeutics for Neurodegenerative Diseases.