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170601s2017 xxu| s |||| 0|eng d |
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|a 9781493970001
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024 |
7 |
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|a 10.1007/978-1-4939-7000-1
|2 doi
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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245 |
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|a Protein Crystallography :
|b Methods and Protocols /
|c edited by Alexander Wlodawer, Zbigniew Dauter, Mariusz Jaskolski.
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250 |
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|a 1st ed. 2017.
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260 |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2017.
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300 |
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|a XIII, 672 p. 173 illus., 145 illus. in color. :
|b online resource.
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336 |
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|a text
|b txt
|2 rdacontent
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|a computer
|b c
|2 rdamedia
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|a online resource
|b cr
|2 rdacarrier
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|a Methods in Molecular Biology,
|v 1607
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|a Expression and Purification of Recombinant Proteins in Escherichia Coli with a His6 or Dual His6-MBP Tag -- Protein Crystallization -- Advanced Methods of Protein Crystallization -- The ‘Sticky Patch’ Model of Crystallization and Modification of Proteins for Enhanced Crystallizability -- Crystallization of Membrane Proteins: and Overview -- Locating and Visualizing Crystals for X-ray Diffraction Experiments -- Collection of X-ray Diffraction Data from Macromolecular Crystals -- Identifying and Overcoming Crystal Pathologies: Disorder and Twinning -- Applications of X-ray Micro-beam for Data Collection -- Serial Synchrotron X-ray Crystallography (SSX) -- Time-Resolved Macromolecular Crystallography at Modern X-ray Sources -- Structure Determination Using X-ray Free-electron Laser Pulses -- Processing of XFEL Data -- Many Ways to Derivatize Macromolecules and their Crystals for Phasing -- Experimental Phasing: Substructure Solution and Density Modification as Implemented in SHELX -- Contemporary Use of Anomalous Diffraction in Biomolecular Structure Analysis -- Long-Wavelength X-ray Diffraction and its Applications in Macromolecular Crystallography -- Acknowledging Errors: Advanced Molecular Replacement with Phaser -- Rosetta Structure Prediction as a Tool for Solving Difficult Molecular Replacement Problems -- Radiation Damage in Macromolecular Crystallography -- Boxes of Model Building and Visualization -- Structure Refinement at Atomic Resolution -- Low Resolution Refinement of Atomic Models against Crystallographic Data -- Stereochemistry and Validation of Macromolecular Structures -- Validation of Protein-Ligand Crystal Structure Models: Small Molecule and Peptide Ligands -- Protein Data Bank - the Single Global Macromolecular Structure Archive Managed by the Worldwide Protein Data Bank -- Databases, Repositories and Other Data Resources in Structural Biology.
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650 |
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|a Proteins .
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650 |
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4 |
|a Protein Science.
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700 |
1 |
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|a Wlodawer, Alexander.
|e editor.
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700 |
1 |
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|a Dauter, Zbigniew.
|e editor.
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700 |
1 |
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|a Jaskolski, Mariusz.
|e editor.
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710 |
2 |
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|a SpringerLink (Online service)
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773 |
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|t Springer eBooks
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