Protein Crystallography : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Wlodawer, Alexander. (Editor ), Dauter, Zbigniew. (Editor ), Jaskolski, Mariusz. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2017.
Edición:1st ed. 2017.
Colección:Methods in Molecular Biology, 1607
Materias:
Tabla de Contenidos:
  • Expression and Purification of Recombinant Proteins in Escherichia Coli with a His6 or Dual His6-MBP Tag
  • Protein Crystallization
  • Advanced Methods of Protein Crystallization
  • The ‘Sticky Patch’ Model of Crystallization and Modification of Proteins for Enhanced Crystallizability
  • Crystallization of Membrane Proteins: and Overview
  • Locating and Visualizing Crystals for X-ray Diffraction Experiments
  • Collection of X-ray Diffraction Data from Macromolecular Crystals
  • Identifying and Overcoming Crystal Pathologies: Disorder and Twinning
  • Applications of X-ray Micro-beam for Data Collection
  • Serial Synchrotron X-ray Crystallography (SSX)
  • Time-Resolved Macromolecular Crystallography at Modern X-ray Sources
  • Structure Determination Using X-ray Free-electron Laser Pulses
  • Processing of XFEL Data
  • Many Ways to Derivatize Macromolecules and their Crystals for Phasing
  • Experimental Phasing: Substructure Solution and Density Modification as Implemented in SHELX
  • Contemporary Use of Anomalous Diffraction in Biomolecular Structure Analysis
  • Long-Wavelength X-ray Diffraction and its Applications in Macromolecular Crystallography
  • Acknowledging Errors: Advanced Molecular Replacement with Phaser
  • Rosetta Structure Prediction as a Tool for Solving Difficult Molecular Replacement Problems
  • Radiation Damage in Macromolecular Crystallography
  • Boxes of Model Building and Visualization
  • Structure Refinement at Atomic Resolution
  • Low Resolution Refinement of Atomic Models against Crystallographic Data
  • Stereochemistry and Validation of Macromolecular Structures
  • Validation of Protein-Ligand Crystal Structure Models: Small Molecule and Peptide Ligands
  • Protein Data Bank - the Single Global Macromolecular Structure Archive Managed by the Worldwide Protein Data Bank
  • Databases, Repositories and Other Data Resources in Structural Biology.