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|a 9781493933754
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|a 10.1007/978-1-4939-3375-4
|2 doi
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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|a Nonribosomal Peptide and Polyketide Biosynthesis :
|b Methods and Protocols /
|c edited by Bradley Evans.
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|a 1st ed. 2016.
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2016.
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|a XI, 281 p. 80 illus., 49 illus. in color. :
|b online resource.
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|a text
|b txt
|2 rdacontent
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|a computer
|b c
|2 rdamedia
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|a online resource
|b cr
|2 rdacarrier
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|a Methods in Molecular Biology,
|v 1401
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|a Structural Biology of Nonribosomal Peptide Synthetases -- The Assembly Line Enzymology of Polyketide Biosynthesis -- Measurement of Nonribosomal Peptide Synthetase Adenylation Domain Activity Using a Continuous Hydroxylamine Release Assay -- Affinity Purification Method for the Identification of Nonribosomal Peptide Biosynthetic Enzymes Using a Synthetic Probe for Adenylation Domains -- Colorimetric Detection of the Adenylation Activity in Nonribosomal Peptide Synthetases -- Facile Synthetic Access to Glycopeptide Antibiotic Precursor Peptides for the Investigation of Cytochrome P450 Action in Glycopeptide Antibiotic Biosynthesis -- Reconstitution of Fungal Nonribosomal Peptide Synthetases in Yeast and In Vitro -- The Continuing Development of E. coli as a Heterologous Host for Complex Natural Product Biosynthesis -- Screening for Expressed Nonribosomal Peptide Synthetases and Polyketide Synthases Using LC‒MS/MS-Based Proteomics -- Enhancing Nonribosomal Peptide Biosynthesis in Filamentous Fungi -- In Situ Analysis of Bacterial Lipopeptide Antibiotics by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging -- Secondary Metabolic Pathway-Targeted Metabolomics -- Annotating and Interpreting Linear and Cyclic Peptide Tandem Mass Spectra -- Bioinformatics Tools for the Discovery of New Nonribosomal Peptides -- The Use of ClusterMine360 for the Analysis of Polyketide and Nonribosomal Peptide Biosynthetic Pathways -- Alignment-Free Methods for the Detection and Specificity Prediction of Adenylation Domains -- Characterization of Nonribosomal Peptide Synthetases with NRPSsp.
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|a Proteins .
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|a Enzymology.
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|a Protein Science.
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|a Enzymology.
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|a Evans, Bradley.
|e editor.
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|a SpringerLink (Online service)
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|t Springer eBooks
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