Nonribosomal Peptide and Polyketide Biosynthesis : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Evans, Bradley. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2016.
Edición:1st ed. 2016.
Colección:Methods in Molecular Biology, 1401
Materias:
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024 7 |a 10.1007/978-1-4939-3375-4  |2 doi 
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245 1 0 |a Nonribosomal Peptide and Polyketide Biosynthesis :  |b Methods and Protocols /  |c edited by Bradley Evans. 
250 |a 1st ed. 2016. 
260 # # |a New York, NY :  |b Springer New York :  |b Imprint: Humana,  |c 2016. 
300 |a XI, 281 p. 80 illus., 49 illus. in color. :  |b online resource. 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
338 |a online resource  |b cr  |2 rdacarrier 
490 1 |a Methods in Molecular Biology,  |v 1401 
505 0 |a Structural Biology of Nonribosomal Peptide Synthetases -- The Assembly Line Enzymology of Polyketide Biosynthesis -- Measurement of Nonribosomal Peptide Synthetase Adenylation Domain Activity Using a Continuous Hydroxylamine Release Assay -- Affinity Purification Method for the Identification of Nonribosomal Peptide Biosynthetic Enzymes Using a Synthetic Probe for Adenylation Domains -- Colorimetric Detection of the Adenylation Activity in Nonribosomal Peptide Synthetases -- Facile Synthetic Access to Glycopeptide Antibiotic Precursor Peptides for the Investigation of Cytochrome P450 Action in Glycopeptide Antibiotic Biosynthesis -- Reconstitution of Fungal Nonribosomal Peptide Synthetases in Yeast and In Vitro -- The Continuing Development of E. coli as a Heterologous Host for Complex Natural Product Biosynthesis -- Screening for Expressed Nonribosomal Peptide Synthetases and Polyketide Synthases Using LC‒MS/MS-Based Proteomics -- Enhancing Nonribosomal Peptide Biosynthesis in Filamentous Fungi -- In Situ Analysis of Bacterial Lipopeptide Antibiotics by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging -- Secondary Metabolic Pathway-Targeted Metabolomics -- Annotating and Interpreting Linear and Cyclic Peptide Tandem Mass Spectra -- Bioinformatics Tools for the Discovery of New Nonribosomal Peptides -- The Use of ClusterMine360 for the Analysis of Polyketide and Nonribosomal Peptide Biosynthetic Pathways -- Alignment-Free Methods for the Detection and Specificity Prediction of Adenylation Domains -- Characterization of Nonribosomal Peptide Synthetases with NRPSsp. 
650 0 |a Proteins . 
650 0 |a Enzymology. 
650 1 4 |a Protein Science. 
650 2 4 |a Enzymology. 
700 1 |a Evans, Bradley.  |e editor. 
710 2 |a SpringerLink (Online service) 
773 0 |t Springer eBooks