Nonribosomal Peptide and Polyketide Biosynthesis : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Evans, Bradley. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2016.
Edición:1st ed. 2016.
Colección:Methods in Molecular Biology, 1401
Materias:
Tabla de Contenidos:
  • Structural Biology of Nonribosomal Peptide Synthetases
  • The Assembly Line Enzymology of Polyketide Biosynthesis
  • Measurement of Nonribosomal Peptide Synthetase Adenylation Domain Activity Using a Continuous Hydroxylamine Release Assay
  • Affinity Purification Method for the Identification of Nonribosomal Peptide Biosynthetic Enzymes Using a Synthetic Probe for Adenylation Domains
  • Colorimetric Detection of the Adenylation Activity in Nonribosomal Peptide Synthetases
  • Facile Synthetic Access to Glycopeptide Antibiotic Precursor Peptides for the Investigation of Cytochrome P450 Action in Glycopeptide Antibiotic Biosynthesis
  • Reconstitution of Fungal Nonribosomal Peptide Synthetases in Yeast and In Vitro
  • The Continuing Development of E. coli as a Heterologous Host for Complex Natural Product Biosynthesis
  • Screening for Expressed Nonribosomal Peptide Synthetases and Polyketide Synthases Using LC‒MS/MS-Based Proteomics
  • Enhancing Nonribosomal Peptide Biosynthesis in Filamentous Fungi
  • In Situ Analysis of Bacterial Lipopeptide Antibiotics by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging
  • Secondary Metabolic Pathway-Targeted Metabolomics
  • Annotating and Interpreting Linear and Cyclic Peptide Tandem Mass Spectra
  • Bioinformatics Tools for the Discovery of New Nonribosomal Peptides
  • The Use of ClusterMine360 for the Analysis of Polyketide and Nonribosomal Peptide Biosynthetic Pathways
  • Alignment-Free Methods for the Detection and Specificity Prediction of Adenylation Domains
  • Characterization of Nonribosomal Peptide Synthetases with NRPSsp.