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100301s1999 xxu| s |||| 0|eng d |
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|a 9781592596768
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| 024 |
7 |
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|a 10.1385/1592596762
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a RNA'Protein Interaction Protocols /
|c edited by Susan R. Haynes.
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| 250 |
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|a 1st ed. 1999.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 1999.
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| 300 |
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|a XIX, 481 p. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 118
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| 505 |
0 |
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|a Labeling and Purification of RNA Synthesized by In Vitro Transcription -- Joining RNA Molecules with T4 DNA Ligase -- RNA-Protein Crosslinking with Photoreactive Nucleotide Analogs -- The Methylene Blue Mediated Photocrosslinking Method for Dectection of Proteins that Interact with Double-Stranded RNA -- Probing RNA-Protein Interactions by Psoralen Photocrosslinking -- Identification and Sequence Analysis of RNA-Protein Contact Sites by N-Terminal Sequencing and MALDI-MS -- RNA Footprinting and Modification Interference Analysis -- Oligonucleotide-Targeted RNase H Protection Analysis of RNA-Protein Complexes -- Nitrocellulose Filter Binding for Determination of Dissociation Constants -- Measuring Equilibrium and Kinetic Constants Using Gel Retardation Assays -- PACE Analysis of RNA-Peptide Interactions -- Detection of Nucleic Acid Interactions Using Surface Plasmon Resonance -- An Escherichia coli-Based Genetic Strategy for Characterizing RNA Binding Proteins -- Screening RNA-Binding Libraries Using a Bacterial Transcription Antitermination Assay -- In Vitro Genetic Analysis of RNA-Binding Proteins Using Phage Display -- In Vitro Selection of Aptamers from RNA Libraries -- Identification of Specific Protein-RNA Target Sites Using Libraries of Natural Sequences -- Northwestern Screening of Expression Libraries -- Screening Expression Libraries with Solution-Based Assay -- An Immunoprecipitation-RNA:rPCR Method for the In Vivo Isolation of Ribonucleoprotein Complexes -- Purification and Depletion of RNP Particles by Antisense Affinity Chromatography -- Purification of U Small Nuclear Ribonucleoprotein Particles -- Preparation of Heterogeneous Nuclear Ribonucleoprotein Complexes -- Preparation of Hela Cell Nuclear and Cytosolic S100 Extracts for In Vitro Splicing -- Mammalian In Vitro Splicing Assays -- Yeast Pre-mRNA Splicing Extracts -- Prespliceosome and Spliceosome Isolation and Analysis -- A Yeast Spliceosome Assay -- Defining Pre-mRNA cis Elements that Regulate Cell-Specific Splicing -- In Vivo SELEX in Vertebrate Cells -- Purification of SR Protein Splicing Factors -- Processing mRNA 3' Ends In Vitro -- Analysis of Poly(A) Tail Lengths by PCR: The PAT Assay -- In Vitro Translation Extracts from Tissue Culture Cells -- Messenger RNA Turnover in Cell-Free Extracts from Higher Eukaryotes.
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| 650 |
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|a Cell biology.
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| 650 |
1 |
4 |
|a Cell Biology.
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| 700 |
1 |
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|a Haynes, Susan R.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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